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核糖核酸与迈格林华尔色素作用机理及含量测定研究
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作者单位
司文会,訾言勤,屠一锋 1.苏州农业职业技术学院2.淮北师范大学化学与材料科学学院3.苏州大学材料与化学化工学部 
基金项目:国家自然科学基金资助项目(20675055);江苏省高校“青蓝工程”中青年学术带头人项目资助
中文摘要:在弱酸性介质中,小分子迈格林华尔色素与生物大分子核糖核酸发生强烈相互作用,导致分子构象变化,进而引起吸收光谱最大波长和吸收值的变化。研究了缓冲溶液酸度、色素用量、反应时间对反应体系的影响,确定了最佳实验条件,建立了测定核糖核酸的新方法。方法的线性范围为0~80 mg/L,相关系数为0999 6,直接测定样品中的核糖核酸含量,结果满意。该文还初步探讨了反应机理,认为静电缔合作用使迈格林华尔色素分子中的正电荷与RNA分子链上的反离子交换,然后以协同方式与RNA分子发生键合,使色素π-电子叠合程度降低,颜色变浅,吸收值降低,最大吸收波长紫移。
中文关键词:核糖核酸  迈格林华尔色素  光谱法  静电缔合
 
Study on the Determination of RNA by Spectrophotometry with May Grunwald′s Stain
Abstract:Based on the fact that both the maximum wavelength of the absorption spectrum of May Grunwald′s stain(MGS) and the corresponding absorbance varied when MGS interacted with the large biological molecules of RNA under weak acidic medium,a novel spectrophotometric method for the determination of RNA was proposed. The effects of the acidity of B-R buffer solution,the amount of MGS,the amount of B-R buffer and the time on the interaction of the system were studied in detail and optimized. The decreased absorbance of MGS has a linear relationship with the content of RNA in the range of 0-08 mg/L and the correlation coefficient was 0999 6. The method is simple,rapid,highly sensitive and selective. It was applied for the determination of RNA in synthetic samples with satisfactory result. The reaction mechanism was preliminary studied.
Key Words:RNA  May Grunwald′s stain  spectrophotometry  electrostatic association
引用本文:司文会,訾言勤,屠一锋.核糖核酸与迈格林华尔色素作用机理及含量测定研究[J].分析测试学报,2011,30(1):24-28.
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