| 液相色谱-串联质谱法测定尿液中的泛酸 |
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| 基金项目:国家自然科学基金项目(21277174) |
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| 中文摘要:该文建立了检测尿液中泛酸含量的液相色谱-串联质谱(HPLC-MS/MS)方法，尿液经过离心、稀释后，采用ACPUITY UPLC SS T3(2.1 mm×100 mm，1.8 μm)色谱柱进行分离，电喷雾正离子模式电离，多反应监测模式进行检测，方法的线性关系良好(r=0.999 3)，方法检出限为0.46 ng/mL，回收率为87.9%~95.3%，相对标准偏差(RSD)为2.5%~13.0%。该方法具有灵敏度高、分析时间短等特点，可用于尿液中泛酸含量的分析。 |
| 中文关键词:液相色谱-串联质谱 尿液 泛酸 生物监测 |
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| Determination of Pantothenic Acid in Human Urine by Liquid Chromatography- Tandem Mass Spectrometry |
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| Abstract:A liquid chromatography-tandem mass spectrometric(HPLC-MS/MS)method for the determination of pantothenic acid in urine was developed and validated.After centrifugation and dilution,the sample was separated on an ACPUITY UPLC HSS T3(2.1 mm×100 mm,1.8 μm) column produced by the Waters corporation,and ionized in electro spray positive ion mode,then detected using multi reaction monitoring mode.The calibration curve has a good linear range with a correlation coefficient(r) of 0.999 3.The limit of detection is 0.46 ng/mL,the recoveries are in the range of 87.9%-95.3%,and the RSDs are in the range of 2.5%-13.0%.The method has the advantages of high sensitivity and short analysis time,and could be used to analyze pantothenic acid in human urine. |
| Key Words:liquid chromatography tandem mass spectrometry urine pantothenic acid biomonitoring |
| 引用本文：张小涛，章云飞，侯宏卫，陈欢，刘勇，王安，胡清源.液相色谱-串联质谱法测定尿液中的泛酸[J].分析测试学报,2017,36(6):809-811. |
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