| 基于MALDI-TOF MS的串联富集策略在磷酸化蛋白组学中的应用 |
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| 基金项目:国家质检总局科技计划项目(2015IK201) |
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| 中文摘要:提出一种除盐-富集串联用于磷酸肽富集研究的思路。选用C18柱和铈(Ⅳ)修饰的壳聚糖材料进行脱盐实验，以制备的基于聚合物基体螯合Fe3+的亲和色谱材料为富集材料。将直接富集和串联策略应用到标准品和血清中，研究结果表明，该富集材料具有高选择性和高灵敏度(1.6 fmol)，铈(Ⅳ)修饰的壳聚糖材料前提下的串联策略能明显降低样品的复杂性。相比直接富集方法，能够提高磷酸化肽的覆盖率。 |
| 中文关键词:脱盐 固定金属离子亲和色谱 基质辅助激光解吸/电离飞行时间质谱 磷酸肽 串联富集 |
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| Application of Tandem Enrichment Strategy by MALDI-TOF MS in Phosphorylated Proteomics |
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| Abstract:A scheme was presented for the tandem purification of phosphopeptide using desalting prior to immobilized metal ion affinity chromatography(IMAC).Both C18 tip and the cerium(Ⅳ)-modified chitosan material(Ce-MCM) were used in the desalting experiments.GMA-co-SR454/Iron(Ⅲ)-IDA was synthesized,and used as an excellent phosphopetides enrichment material.They were applied to selectively capture the phosphopeptides in standards and human serum.Results demonstrated that C18/IMAC and Ce-MCM/IMAC have different performances because of their hydrophobic or hydrophilic properties.Ce-MCM/IMAC tandem strategy notably decreased the complexity of peptides,and yielded a far greater coverage for phosphopeptides than single IMAC. |
| Key Words:desalting IMAC matrix-assisted laser desorption-ionization time of flight mass spectrometry(MALDI-TOF MS) phosphopeptides tandem enrichment |
| 引用本文：张传静，吴婷，任红鑫，张玲帆，杜一平.基于MALDI-TOF MS的串联富集策略在磷酸化蛋白组学中的应用[J].分析测试学报,2017,36(9):1061-1068. |
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