| 高效液相色谱-电化学检测法研究大蒜素对百草枯中毒小鼠脑组织中单胺类神经递质含量的影响 |
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| 基金项目:国家自然科学基金资助项目(81573202) |
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| 中文摘要:建立了单胺类神经递质的高效液相色谱-电化学检测方法，探讨了大蒜素对百草枯中毒小鼠脑组织内的单胺类神经递质及其代谢产物的影响。健康ICR小鼠随机分为4组：正常对照(Con)组、大蒜素(DAS)组、百草枯染毒(PQ)组和大蒜素治疗(PQ+DAS)组，每组6只。PQ组灌胃PQ 35mg/kg建立中毒模型，Con组和DAS组灌服同体积生理盐水。DAS组和PQ+DAS组小鼠于造模2、26、50h后腹腔内各注射1次DAS(25mg/kg)，PQ组和正常组小鼠腹腔内注射等量无菌生理盐水。造模12h后处死小鼠。收集小鼠脑组织样品并称重，处理后进样。色谱分析采用 Acclaim 120 C18(150mm×2.1mm，3μm)色谱柱，以离子对缓冲盐溶液-甲醇(93∶7，体积比)为流动相，流速为0.27mL/min；电化学检测器检测，工作电压为450mV。结果表明，与正常组和DAS组比较，PQ组部分神经递质水平降低；DAS治疗后能够改变上述生化指标的变化；与PQ组相比，PQ+DAS组神经递质含量增高，差异均具有统计学意义(P<0.05)。脑组织样品中5-HT、DA、5-HIAA、DOPAC和HVA低、中、高3水平的加标回收率均为79.8%~112.1%，相对标准偏差(RSD)小于10%。实验表明，大蒜素对百草枯中毒后小鼠体内含量降低的神经递质有明显的上调作用。 |
| 中文关键词:高效液相色谱-电化学检测 百草枯 大蒜素 单胺类神经递质 |
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| Study on Effects of Alltride on Monoamine Neurotransmitters in Brain of Paraquat Poisoning Rats by HPLC-ECD |
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| Abstract:An HPLC-ECD method was established for the determination of five neurotransmitters in cerebral tissues of rats to discuss the protective effects of alltride(DAS) on the monoamine neurotransmitters(MNTs) in paraquat(PQ) poisoning rats brain tissues. Male ICR rats were divided into four groups(n=6) according to their different treatments. The paraquat(PQ) group and the treatment(PQ+DAS) group exposed to 35mg/kg PQ i.g.. The control group and the alltride(DAS) group were given the same volume of sterile saline. The alltride(DAS) group and treatment group were administrated with 25mg/kg DAS i.p. after PQ was given intragastrically for 2,26,50 h.PQ group and normal group rats were injected with the same amount of sterile saline. The determination was performed on an Acclaim 120 C18 column with a mobile phase consisted of buffer salt(sodium citrate,monopotassium phosphate,sodium heptanesulfonate,EDTA-2Na,diethylamine hydrochloride )-methanol(93∶7,by volume) at a flow rate of 0.27mL/min,and the voltage of electrode was set at 450mV.Compared with the normal or DAS group，the PQ group showed significant decreases in the quantities of DA and 5-HT.The combination of PQ and DAS resulted in higher amounts of neurotransmitters than that of the administration with PQ alone(P<0.05).The spiked recoveries were in the range of 79.8%-112.1% with RSDs lower than 10%.DAS could prevent rats from the PQ-induced decrease of the neurotransmitters, which may be associated with its abilities to relieve oxidative damages. |
| Key Words:HPLC-ECD paraquat alltride monoamine neurotransmitters |
| 引用本文：檀笑昕，张淑娟，刘坤，张铧尹，连靠奇，徐向东，康维钧.高效液相色谱-电化学检测法研究大蒜素对百草枯中毒小鼠脑组织中单胺类神经递质含量的影响[J].分析测试学报,2017,36(9):1069-1074. |
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