| 纳米金-还原氧化石墨烯修饰葡萄糖氧化酶传感器的制备及其电流法检测饮料中的葡萄糖 |
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| 基金项目:安徽省自然科学基金项目(1508085MC42，1508085MC47)；国家自然科学基金青年科学基金项目(31401573)；安徽省科技计划项目(1604g0702051) |
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| 中文摘要:利用纳米金(AuNPs)与还原氧化石墨烯(rGO)复合纳米材料制备了葡萄糖氧化酶生物传感器并用于饮料中葡萄糖含量的检测。将壳聚糖作为还原剂及稳定剂，通过一步法合成了AuNPs-rGO复合材料，并通过物理吸附固定葡萄糖氧化酶(GOx)来制作GOx生物传感器。该传感器在磷酸盐缓冲溶液(0.1 mol/L，pH 6.0)中，-0.45 V(vs.Ag/AgCl)电位下电流法检测葡萄糖含量，线性检测范围为0.01~0.88 mmol/L，灵敏度为22.54 μA?mmol-1?L?cm-2，检出限为1.01 μmol/L，且表观米氏常数为0.497 mmol/L。该传感器用于多种饮料中葡萄糖含量的直接检测，结果满意。 |
| 中文关键词:纳米金 还原氧化石墨烯 葡萄糖氧化酶 电流法 饮料 葡萄糖 |
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| Preparation of a Gold Nanoparticles-Reduced Graphene Oxide Modified Glucose Oxidase Biosensor and Its Amperometric Determination of Glucose in Beverages |
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| Abstract:An amperometric glucose oxidase(GOx) biosensor based on gold nanoparticles(AuNPs) -reduced graphene oxide(rGO) was fabricated for the detection of glucose in beverages.AuNPs-rGO composite was synthesized by one-pot method with chitosan as reducing and stabilizing agent.It was used as the matrix for GOx immobilization via physical adsorption technique to fabricate the GOx-based biosensor.The biosensor was applied in the amperometric determination of glucose at -0.45 V(vs.Ag/AgCl) in a phosphate buffer (0.1 mol/L，pH 6.0).There was a linear response to glucose in the concentration range of 0.01-0.88 mmol/L,with a sensitivity of 22.54 μA?mmol-1?L?cm-2 and a detection limit of 1.01 μmol/L.The apparent Michaelis-Menten constant was rather small(0.497 mmol/L).The constructed biosensor was successfully applied in the detection of glucose in beverages. |
| Key Words:gold nanoparticles reduced graphene oxide glucose oxidase amperometric determination beverage glucose |
| 引用本文：郑海松，毛慎，丁顺，陈雪娇，李云飞，宗凯，操小栋，叶永康.纳米金-还原氧化石墨烯修饰葡萄糖氧化酶传感器的制备及其电流法检测饮料中的葡萄糖[J].分析测试学报,2017,36(9):1114-1118. |
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