| 金标记羟胺放大化学发光检测赭曲霉毒素A |
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| 基金项目:国家自然科学基金项目(31660491)；江西省自然科学基金项目(20132BAB205106) |
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| 中文摘要:以羧基磁性微球为分离载体,连接氨基捕获探针和适配体,加入生物素化报告序列和赭曲霉毒素A(Ochratoxin A，OTA)竞争结合适体,继续加入链霉亲和素纳米金和羟胺/Au3+以显著提高化学发光检测OTA的灵敏度，从而建立了一种纳米金标记羟胺放大化学发光检测OTA的高灵敏度方法。优化了羧基磁性微球、氨基捕获探针、适配体、生物素化报告序列、链霉亲和素纳米金的用量。优化条件下，在OTA质量浓度0.01~50 ng/mL范围内，化学发光信号值与OTA浓度的对数呈较好的线性关系(r2=0.992 5)，检出限为1.58×10-3 ng/mL。对啤酒样品进行OTA加标回收实验，回收率为97.4%~105.4%，相对标准偏差为4.0%~5.5%。 |
| 中文关键词:赭曲霉毒素A 链霉亲和素纳米金 羟胺 化学发光 |
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| Detection of Ochratoxin A by Hydroxylamine-amplified Gold Nanoparticles Chemiluminescence |
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| Abstract:A novel chemiluminescent(CL) aptasensor for ochratoxin A(OTA) with magnetic beads as separate carrier was designed.Firstly,the capture DNA and OTA aptamer were immobilized on the surface of the magnetic beads,then the biotin-reporter DNA and OTA were taken to competitively bind to the OTA aptamer.Finally,streptavidin-gold nanoparticles(SA-AuNPs) and NH2OH/Au3+ were added sequencely in order to improve the sensitivity for CL detection on OTA.The effects on the experiment including magnetic beads，capture DNA，aptamers，biotin-reporter DNA and dilution multiple of SA-AuNPs,were optimized.Under the optimized experimental conditions,there existed a good linear relationship between the CL intensity(ΔCL) and the logarithms of OTA concentration(r2=0.992 5) in the range of 0.01-50 ng/mL,with a detection limit of 1.58×10-3 ng/mL.In the recovery experiments by adding different concentrations of OTA to the beer samples,the recoveries ranged from 97.4% to 105.4% with the relative standard deviations of 4.0%-5.5%. |
| Key Words:ochratoxin A SA-AuNPs hydroxylamine chemiluminescence |
| 引用本文：李素,肖义陂,武乐,姜萌萌,刘杰,严喜鸾.金标记羟胺放大化学发光检测赭曲霉毒素A[J].分析测试学报,2018,37(1):57-61. |
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