| 基于不同电化学探针检测乳腺癌基因片段电化学传感器的研究 |
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| 基金项目:延安大学科研项目(YD2014-04) |
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| 中文摘要:构建了以3种不同电活性物质(铁氰化钾平衡电对、亚甲基蓝和六氨合钌)为电化学信号探针，检测乳腺癌基因片段(乳腺癌DNA)的电化学传感器。利用吸附作用将探针ssDNA固定于金纳米-多壁碳纳米管-Nafion复合纳米材料修饰金电极表面，制备了DNA电化学传感器。采用循环伏安法、电化学阻抗法和微分脉冲伏安法，对DNA电化学传感器进行表征和定量分析。实验结果表明，在5 mmol/L K3［Fe(CN)6］ -5 mmol/L K4［Fe(CN)6］平衡电对电化学探针检测液中，乳腺癌DNA的线性范围为0.1~500.0 nmol/L，其检出限(S/N=3)为0.03 nmol/L。以20 μmol/L亚甲基蓝为电化学探针检测液时，乳腺癌DNA的线性范围为1.0~500.0 nmol/L，检出限为0.3 nmol/L。利用50 μmol/L六氨合钌电化学探针检测时，乳腺癌DNA的线性范围为1.0~500.0 nmol/L，检出限为0.3 nmol/L。3种电化学探针中，利用铁氰化钾平衡电对探针检测乳腺癌DNA的检出限最低，线性范围最宽。该传感器可用于其他DNA的检测分析。 |
| 中文关键词:电化学传感器 乳腺癌基因片段 电化学探针 多壁碳纳米管 金纳米 Nafion |
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| Determination of Breast Cancer Genes Using an Electrochemical DNA Sensor Based on Three Different Probes |
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| Abstract:A novel DNA sensor was designed for the determination of breast cancer genes based on three electrochemical probes,the negative electrochemical probe ［Fe(CN)6］3-/4-,methylene blue and the positive electrochemical probe［Ru(NH3)6］3+.The breast cancer genes DNA sensor was fabricated by immobilizing a breast cancer gene ssDNA onto the surface of gold nanoparticles-multi-walled carbon nanotubes(MWCNT)-Nafion modified gold electrode through adsorption,and characterized by cyclic voltammetry,differential pulse voltammetry and electrochemical impedance method.Under the optimized conditions,in 5 mmol/L K3［Fe(CN)6］ -5 mmol/L K4［Fe(CN)6］(pH=7.4) solution,the electrochemical current response of the sensor was linear with the logarithm of the breast cancer gene concentration in range of 0.1-500.0 nmol/L,with a detection limit(S/N=3) of 0.03 nmol/L.While using methylene blue as the electrochemical probe,the electrochemical current response was linear with concentration of breast cancer gene in range of 1.0-500.0 nmol/L,and the detection limit was 0.3 nmol/L.Based on the different binding ability of ［Ru(NH3)6］3+with ssDNA and dsDNA,the current response of the sensor using ［Ru(NH3)6］3+ as the probe was still linear with concentration of breast cancer gene in the range of 1.0-500.0 nmol/L.The detection limit was 0.3 nmol/L.Among three kinds of electrochemical probes,［Fe(CN)6］3-/4- probe had the lowest detection limit and the widest linear range for the detection of breast cancer gene. |
| Key Words:electrochemical biosensor breast cancer gene electrochemical probe multi-walled carbon nanotubes gold nanoparticles Nafion |
| 引用本文：贺彩梅，胡晓琴，刘舒萍，李晓霞.基于不同电化学探针检测乳腺癌基因片段电化学传感器的研究[J].分析测试学报,2018,37(3):269-274. |
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