| U-PLS/RBL三维荧光法测定藤络宁胶囊中东莨菪内酯含量 |
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| 基金项目:国家自然科学基金资助项目(20975029,81173496) |
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| 中文摘要:研究了东莨菪内酯的荧光性质和最佳实验条件，发现体系的最佳pH值为9.0，最大激发波长为375 nm，最大发射波长为460 nm。在激发波长310~420 nm，发射波长400~550 nm范围内采集实际样本的三维荧光数据矩阵。采用二阶校正中的U-PLS/RBL(Unfolded partial least squares coupled to residual bilinearization)解析三维荧光数据阵，成功地测定了藤络宁胶囊中的东莨菪内酯含量。用U-PLS/RBL解析藤络宁胶囊中的东莨菪内酯含量，结果与HPLC方法一致，说明荧光解析法结果准确可靠。用U-PLS/RBL解析藤络宁胶囊样品的加标回收率为100.4%。相比于色谱方法,荧光方法具有操作费用低及绿色环保等优点。 |
| 中文关键词:U-PLS/RBL 东莨菪内酯 藤络宁胶囊 高效液相色谱 三维荧光 |
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| Determination of Scopoletin in Tengluoning Capsule Using Three-dimensional Fluorescence Combined with Unfolded Partial Least Squares Coupled to Residual Bilinearization(U-PLS/RBL) |
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| Abstract:A method for the determination of scopoletin in Tengluoning capsule by three-dimensional fluorescence combined with unfolded partial least squares coupled to residual bilinearization(U-PLS/RBL) was developed.Experiments showed that scopoletin has a strong fluorescent signal.The optimum pH of the system was 9.0,the maximum excitation wavelength of scopoletin was 375 nm,and the maximum emission wavelength was 460 nm.Excitation-emission matrices(EEM) of Tengluoning capsule samples were obtained,recording emission between 400 nm and 550 nm,exciting between 310 nm and 420 nm,for the analysis of scopoletin.An effective second-order calibration method,U-PLS/RBL(unfolded partial least-squares coupled to residual bilinearization) was used to analyze the three-dimensional fluorescent data array,and the content of scopoletin was successfully determined.The predicted U-PLS/RBL concentrations were satisfactory compared with those obtained by high performance liquid chromatography with fluorescence detection.The average recovery by U-PLS/RBL was 100.4%，which indicated that the proposed method was reliable.Compared to chromatographic method,the fluorescent method has the advantages of low operating cost and pro-environment. |
| Key Words:U-PLS/RBL scopoletin Tengluoning capsule HPLC three-dimensional fluorescence |
| 引用本文：刘铁，刘德龙,魏永巨.U-PLS/RBL三维荧光法测定藤络宁胶囊中东莨菪内酯含量[J].分析测试学报,2018,37(3):313-318. |
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