| 高效液相色谱法测定食品中的爱德万甜 |
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| 中文摘要:建立了食品中爱德万甜检测的高效液相色谱分析方法。样品经甲醇-水（50∶50，体积比）溶液提取10 min，离心后取上清液进行分析。采用Agilent TC-C18（2）（250 mm×46 mm,5 μm）色谱柱分离，以甲醇-002 mol/L乙酸铵（53∶47,体积比）等度洗脱，在280 nm波长下检测，外标法定量。结果表明，目标化合物在10~2000 mg/L范围内呈良好的线性，相关系数（r）为0999 8，饮料、酸奶和蛋糕的检出限（S/N≥3）分别为06、15、90 mg/kg。方法的回收率为909%~1034%，相对标准偏差（RSD，n=5）为11%~45%。该方法的色谱分离效果好，干扰少，准确度高，可用于实际样品中爱德万甜的测定。 |
| 中文关键词:高效液相色谱法 测定 食品 爱德万甜 |
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| Determination of Advantame in Foods by High Performance Liquid Chromatography |
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| Abstract:A method for the determination of advantame in foods using high performance liquid chromatography（HPLC） was developed.The samples were extracted with methanol-water（50∶50,by volume） in ultrasonic condition for 10 min.The supernatant was analyzed after centrifugation.Target compound was separated on an Agilent TC-C18（2）（250 mm×46 mm,5 μm） column at 280 nm,with methanol-002 mol/L ammonium acetate（53∶47,by volume） as mobile phase by isocratic elution.The quantitative analysis was carried out by the external standard method.The results showed that the calibration curves were linear in the range of 10-2000 mg/L with correlation coefficients of 0999 8.The limits of detection（S/N≥3） of the method for the analytes in beverage,yoghourt and cake were 06，15 and 90 mg/kg,respectively.The average recoveries ranged from 909% to 1034% with relative standard deviations（RSDs,n=5） of 11%-45%.With the advantages of good separation,few impurity disturbances and high accuracy,this method could be applied in the analysis of advantame in real samples. |
| Key Words:high performance liquid chromatography（HPLC） determination food advantame |
| 引用本文：汪辉，曹阳，曹雄杰，曹雯婷.高效液相色谱法测定食品中的爱德万甜[J].分析测试学报,2018,37(5):635-638. |
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