| 超高效液相色谱-串联质谱法快速检测蔬菜中23种植物生长调节剂 |
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| 基金项目:国家农产品质量安全风险评估项目（GJFP201700202） |
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| 中文摘要:建立了超高效液相色谱-串联质谱（UHPLC-MS/MS）快速检测蔬菜中23种植物生长调节剂的分析方法。蔬菜样品采用含1%（体积分数）乙酸的乙腈提取，6 g无水硫酸镁和1 g氯化钠盐析后，无需净化，直接进行UHPLC-MS/MS分析，正负离子同时扫描和多反应监测模式（MRM）检测，基质匹配标准溶液外标法定量。23种植物生长调节剂在各自的浓度范围内线性关系良好，相关系数（r2）均大于0.98。除矮壮素、缩节胺的回收率为50.5%~73.7%，其余21种植物生长调节剂在3个加标水平下的平均回收率为70.2%~125.6%，相对标准偏差（RSD）为0.7%~22.5%，方法定量下限为 1~50 μg/kg。该方法简单、快速、灵敏、准确，适用于蔬菜样品中23种植物生长调节剂的快速检测。 |
| 中文关键词:超高效液相色谱-串联质谱（UHPLC-MS/MS） 植物生长调节剂 蔬菜 |
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| Rapid Detection of 23 Plant Growth Regulators in Vegetables by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| Abstract:A rapid method was developed for the simultaneous determinaion of 23 plant growth regulators in vegetables by ultra high performance liquid chromatography-tandem mass spectrometry（UHPLC-MS/MS）.The vegetable samples were extracted with acetonitrile containing 1% acetic acid，followed by salting out with 6 g magnesium sulfate and 1 g sodium chloride，then the solvent extracts were analyzed by UHPLC-MS/MS without further purification.The positive and negative modes and multiple reaction monitoring（MRM） mode were used for identification while the matrix-matched external standard calibration curves were used for quantification.The results showed that there existed good linearities for 23 plant growth regulators in corresponding concentration ranges with their correlation coefficients（r2） higher than 0.98.The average recoveries for 21 plant growth regulators at three spiked levels ranged from 70.2% to 125.6% except that those for chlormequat and mepiquat chloride were from 50.5% to 73.7%，with their relative standard deviations（RSDs） of 0.7%-22.5%.The limits of quantitation of this method were in the range of 1-50 μg/kg.The method is simple，rapid，sensitive and accurate，and is suitable for the rapid qualitation and quantitation for 23 plant growth regulators in vegetables. |
| Key Words:ultra high performance liquid chromatography-tandem mass spectrometry（UHPLC-MS/MS） plant growth regulators vegetables |
| 引用本文：邱世婷，韩梅，侯雪，郭灵安，雷绍荣，陈巧.超高效液相色谱-串联质谱法快速检测蔬菜中23种植物生长调节剂[J].分析测试学报,2018,37(6):660-668. |
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