| 免标记自增强电化学发光免疫传感器超灵敏检测河豚毒素 |
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| 基金项目:浙江省自然科学基金公益技术项目（LGF18B070002）；宁波市自然科学基金（2017A610231）；宁波大学王宽诚幸福基金资助项目 |
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| 中文摘要:以Nafion固定钌联吡啶Au纳米颗粒（Ru（bpy）2+3-AuNPs）于玻碳电极（GCE），借助Au—N共价键固定河豚毒素抗体（anti-TTX），通过抗原抗体的特异性结合构建了超灵敏检测河豚毒素（TTX）的免标记自增强电化学发光（ECL）免疫传感器。当目标物TTX被特异性捕获到修饰电极表面后，可以作为Ru（bpy）2+3的共反应物增强ECL信号。与共反应物存在于溶液中或标记于二抗上相比，其优点在于发光体和共反应物之间的电子转移距离更短，可有效改善电化学发光效率，降低实验成本。最佳条件下，TTX质量浓度在0.01~1 000 μg·L-1范围内与ECL信号呈良好的线性关系，检出限（LOD）为0.01 μg·L-1。此免标记自增强型ECL免疫传感器显示出优异的稳定性、重现性和灵敏度，对实际样品中TTX的回收率为98.0%~104.0%，表明其具有较好的实用价值。 |
| 中文关键词:电化学发光 免疫传感器 河豚毒素 Nafion-Ru（bpy）2+3-AuNPs复合物 |
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| A Label-free Self-enhanced Electrochemiluminescence Immunosensor for Ultrasensitive Detection of Tetrodotoxin |
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| Abstract:A label-free self-enhanced electrochemiluminescence（ECL） immunosensor was fabricated for the ultrasensitive detection of tetrodotoxin（TTX） based on immobilizing tris（2,2′-bipyridyl） ruthenium（Ⅱ）-Au nanoparticles（Ru（bpy）2+3-AuNPs） composites to glassy carbon electrode（GCE） with Nafion.In this strategy,an anti-TTX was able to fix on the electrode surface via Au-N chemical bond for realizing the specific capture toward the target.Meanwhile,TTX could be used as the co-reactant of Ru（bpy）2+3 to promote the ECL intensity.The advantage of this approach is that the electron transfer distance between the emitter and the co reactant is shorter,and the ECL efficiency is improved and the experimental cost is reduced compared with that in the presence of co-reactant in solution or labeling of second antibody.Under the optimum conditions,the change of ECL intensity demonstrated a linear relationship with TTX concentration in the range of 0.01-1 000 μg·L-1,with a limit of detection（LOD） down to 0.01 μg·L-1.This label-free self-enhanced ECL immunosensor exhibited an excellent performance with good stability,reproducibility and sensitivity.The recoveries for TTX in real samples were in the range of 98.0%-104.0%,indicated it has good practical value. |
| Key Words:electrochemiluminescence immunosensor tetrodotoxin Nafion-Ru（bpy）2+3-Au composite |
| 引用本文：刘媛，王邃.免标记自增强电化学发光免疫传感器超灵敏检测河豚毒素[J].分析测试学报,2018,37(6):676-681. |
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