| 黄芩素室温磷光传感器的构建及应用 |
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| 基金项目:山西省软科学研究项目（2014041020-2）；山西师范大学生命科学学院基金（SMYKE-43，SMYKZ-13）；山西师范大学大学生创新创业项目（SD2014CXXM-12） |
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| 中文摘要:黄芩素可通过电子转移效应猝灭聚乙烯亚胺（PEI）包覆的锰（Mn）掺杂硫化锌（ZnS）量子点（PEI-Mn/ZnS QDs）的室温磷光。基于上述原理，建立了一种简单、快速测定黄芩素含量的室温磷光检测方法，PEI-Mn/ZnS QDs以水相共沉淀法获得。结果表明：当pH＝80,反应时间为10 min时，量子点的磷光猝灭程度与黄芩素浓度呈良好的线性关系，线性范围为007 ~060 mg·L-1，方法检出限为0039 mg·L-1，相关系数（r）为0997，相对标准偏差为41%，实际样品的检测回收率为960%~1007%。所建立的方法可有效避免常见离子和氨基酸等的干扰，适用于黄芩饮片及血清中黄芩素含量的快速测定，有望应用于医药行业中黄芩素含量的测定分析，也为其他药物基于量子点电子转移机制的检测提供了技术依据。 |
| 中文关键词:量子点；室温磷光检测；黄芩素；黄芩饮片；血清 传感器 |
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| Construction and Application of A Room temperature Phosphorescence Sensor for Baicalein |
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| Abstract:A relatively simple and fast phosphorescence method for the detection of baicalein（BAI） was established,based on that baicalein could quench the room temperature phosphorescence（RTP） of PEI-Mn/ZnS quantum dots（QDs） prepared by water phase coprecipitation through electron transfer effect.The experimental results showed that,at pH 80 and a reaction time of 10 min,the phosphorescence quenching degree of QDs had a linear relationship（r=0997） with BAI in the concentration range of 007-060 mg·L-1.The detection limits of the method were 0039 mg·L-1,and the relative standard deviations were 41%.The spiked recoveries for practical samples were in the range of 960%-1007%.The established method,effectively avoiding the interference of common ions and amino acids etc., could be used for the rapid determination of BAI in scutellaria baicalensis georgi and serum,which is expected to be applied in the determination and analysis of BAI in medicine industry.It also provides a technical basis for the detection of other drugs based on the mechanism of QDs electron transfer. |
| Key Words:quantum dots（QDs） room temperature phosphorescence（RTP） baicalein（BAI） scutellaria baicalensis georgi serum sensor |
| 引用本文：郭艳霞,王媛媛,李淑娴,王文科.黄芩素室温磷光传感器的构建及应用[J].分析测试学报,2018,37(7):784-789. |
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