| 鲜湿米粉中两种乳酸链球菌素的HPLC-MS/MS检测方法 |
| 点此下载全文 |
|
| 基金项目:食品安全地方标准制定(修订)项目(桂地标食2017006号)；食品安全地方标准制定(修订)项目(桂地标食2017007号)；广西食品药品监督管理局研究项目(桂食药科2018-7) |
|
| 中文摘要:建立了高效液相色谱-串联质谱(HPLC-MS/MS)同时测定鲜湿米粉中乳酸链球菌素A(Nisin A)和乳酸链球菌素Z(Nisin Z)的分析方法。样品经pH 30的水(甲酸调节pH值)提取，以Hilic Plus色谱柱分离待测物，在电喷雾正离子化模式下，采用多反应监测(MRM)模式检测。结果表明，Nisin A和Nisin Z在100~2 000 μg/L范围内线性关系良好，相关系数均大于0999，方法检出限分别为001、002 mg/kg，回收率为956%~1070%，RSD均小于20%。该方法准确、可靠，适用于鲜湿米粉中Nisin A和Nisin Z的含量检测。 |
| 中文关键词:乳酸链球菌素A(Nisin A) 乳酸链球菌素Z(Nisin Z) 高效液相色谱-串联质谱(HPLC-MS/MS) 鲜湿米粉 |
| |
| Determination of Nisin A and Nisin Z in Fresh Wet Rice Noodles by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
|
|
| Abstract:A high performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS) method was developed for the simultaneous analysis of Nisin A and Nisin Z in fresh wet rice noodles.An extraction solution of formic acid(pH 30) was used to extract Nisin A and Nisin Z in fresh wet rice noodles samples.The extract was separated on a Hilic column,and determined with electrospray ionization(ESI) source in positive ion mode under multiple reaction monitoring(MRM) mode.There were good linear relationships for the analytes between their peak area responses and concentrations of 100-2 000 μg/L,with correlative coefficients larger than 0999.The limits of detection for Nisin A and Nisin Z were 001 mg/kg and 002 mg/kg,respectively.The recoveries were in the range of 956%-1070% with RSDs less than 20%.The method was reliable and accurate,and was suitable for the determination of Nisin A and Nisin Z in fresh wet rice noodles. |
| Key Words:Nisin A Nisin Z HPLC-MS/MS fresh wet rice noodles |
| 引用本文：刘珈伶，廖强，蒋定之，陈宁周.鲜湿米粉中两种乳酸链球菌素的HPLC-MS/MS检测方法[J].分析测试学报,2018,37(7):820-824. |
| 摘要点击次数: 1897 |
| 全文下载次数: 800 |
| 查看全文 下载PDF阅读器 |
|
|
