高效毛细管电泳法测定泳池中尿液指示物乙酰磺胺酸钾 |
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基金项目:国家自然科学基金资助项目(81402723);河北省自然科学基金项目(H2018206297) |
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中文摘要:建立了高效毛细管电泳测定游泳池水中新型尿液指示物乙酰磺胺酸钾含量的新方法。采用内壁无涂层熔融石英毛细管(602 cm × 75 μm,有效长度50 cm)进行分离,缓冲液为10 mmol/L硼砂溶液(pH 93),分离电压为24 kV,进样时间为20 s,检测波长为226 nm。取游泳池水样过滤,经固相萃取小柱浓缩富集后直接进样分析。结果显示:在优化条件下,乙酰磺胺酸钾在02~1000 mg/L质量浓度范围内线性良好(r=0999 8),检出限为500 μg/L,迁移时间和峰高的相对标准偏差(RSD)分别为073%和18%,加标回收率为960%~1036 %。该方法简单快速、准确可靠,适用于游泳池水中乙酰磺胺酸钾的检测。 |
中文关键词:高效毛细管电泳法 固相萃取 乙酰磺胺酸钾 尿液指示物 |
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Determination of Acesulfame Potassium in Swimming Pool by High Performance Capillary Electrophoresis |
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Abstract:A high performance capillary electrophoresis method with solid phase extraction was established for the determination of acesulfame potassium(ACE),a urine indicator in swimming pool water.The separation was performed on a fused silica capillary with uncoated inner wall(602 cm×75 μm id.,an effective length of 50 cm),using 10 mmol/L sodium tetraborate(pH 93) as electrophoresis buffer at a separation voltage of 24 kV.The sample was injected for 20 seconds at a detection wavelength of 226 nm.The swimming pool samples were directly analyzed after filtered and enriched by solid phase extraction(SPE).The results showed that,under the optimized conditions,a good linear relationship was obtained for acesulfame potassium in the concentration range of 02-1000 mg/L,with a correlation coefficient(r) of 0999 8,and a detection limit of 500 μg/L.The spiked recoveries were in the range of 960%-1036% with relative standard deviations(RSDs) for migration time and peak height of 073% and 18%,respectively.The method is sensitive,simple and reliable,and is suitable for the detection of acesulfame potassium in swimming pool water. |
Key Words:high performance capillary electrophoresis solid phase extraction acesulfame potassium urine indicator |
引用本文:韩燕祯,朱怀娇,石红梅,徐向东.高效毛细管电泳法测定泳池中尿液指示物乙酰磺胺酸钾[J].分析测试学报,2018,37(7):854-857. |
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