| 基于多巴胺-氧化石墨烯复合物的搅拌棒吸附萃取/高效液相色谱法测定食用油中黄曲霉毒素 |
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| 基金项目:河北省高等学校科学技术研究项目（QN2017106） |
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| 中文摘要:建立了一种基于贻贝仿生化学涂层的搅拌棒吸附萃取/高效液相色谱/荧光检测器（SBSE/HPLC FLD）同时测定食用油中黄曲霉毒素B1、B2、G1、G2的方法。基于贻贝仿生化学制备多巴胺-氧化石墨烯复合物固相萃取材料，利用搅拌棒吸附萃取技术对样品进行提取；以甲醇-乙腈-水（10%磷酸调至pH 35，体积比3∶3∶5）作为流动相，采用荧光检测器进行检测。结果显示，黄曲霉毒素B1、B2、G1、G2在0200~100 μg/L范围内具有良好的线性关系（相关系数r2≥0998 9），加标回收率为815%~969%，日内相对标准偏差（RSD）为17%~34%，日间RSD为19%~35%，方法检出限为0025~0050 μg/L。该方法高效、灵敏、可靠，能够满足食用油中黄曲霉毒素B1、B2、G1、G2的测定要求。 |
| 中文关键词:贻贝仿生化学 多巴胺-氧化石墨烯复合物 搅拌棒吸附萃取 高效液相色谱/荧光检测器 食用油 黄曲霉毒素 |
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| Determination of Aflatoxins in Edible Oil by High Performance Liquid Chromatography with Stirring Bar Sorptive Extraction Based on Dopamine-Graphene Oxide Complex |
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| Abstract:A method of stirring bar sorptive extraction（SBSE） based on mussel-bioinspired chemistry coating and high performance liquid chromatography with fluorescence detector（HPLC/FLD） was developed for the simultaneous determination of aflatoxin B1，B2，G1 and G2（AFB1，AFB2，AFG1 and AFG2） in edible oil.The sample was extracted by SBSE with dopamine-graphene oxide complex as solid phase extraction material prepared by mussel-bioinspired chemistry.The target compounds were separated on a Kromasil C18 column using methanol-acetonitrile-water aqueous solution（3∶3∶5 by volume，adjust pH 35 with 10%phosphoric acid） as mobile phases，and detected with a fluorescence detector.The calibration curves for AFB1，AFB2，AFG1 and AFG2 were linear in the concentration range of 0200-100 μg/L，with their correlation coefficients（r2） not less than 0998 9.The spkied recoveries for 4 aflatoxins（AFs） in edible oil samples ranged from 815% to 969%，with intra day relative standard deviations（RSDs） of 17%-34% and inter day RSDs of 19%-35%.The limits of detection（LODs） were in the range of 0025-0050 μg/L.The proposed method is efficient，sensitive and reliable，and is suitable for the determination of AFB1，AFB2，AFG1 and AFG2 in edible oil. |
| Key Words:mussel-bioinspired chemistry dopamine-graphene oxide complex stirring bar sorptive extraction（SBSE） high performance liquid chromatography with fluorescence detector（HPLC/FLD） edible oil aflatoxin
（AF） |
| 引用本文：李珺沬，律涛，刘彦，李程，王新宇，马海燕.基于多巴胺-氧化石墨烯复合物的搅拌棒吸附萃取/高效液相色谱法测定食用油中黄曲霉毒素[J].分析测试学报,2018,37(8):913-918. |
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