| HPLC测定中药渣中人参皂苷Rg1、Re与Rb1含量 |
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| 基金项目:湖南省战略性新兴产业科技攻关项目（2014GK1007）；中央驻湘科研机构技术创新发展专项（2013TF3006）；中国工程院咨询研究项目（2015-XY-41） |
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| 中文摘要:建立了一种利用反相高效液相色谱检测中药渣中人参皂苷Rg1、Re 和 Rb1含量的分析方法。考察了提取试剂、提取温度、提取溶剂倍量、提取时间对提取效果的影响，最终将样品以10倍体积的70%甲醇于40 ℃水浴中超声提取05 h。采用Zorbax SB-C18（46 mm×150 mm,5 μm）柱分离，以乙腈和水为流动相进行梯度洗脱，流速为10 mL/min，进样量为20 μL，柱温为20 ℃，检测波长为203 nm，采用外标法定量。在该条件下，人参皂苷Rg1、Re、Rb1分别在1874~25761、3748~46854、388~9693 mg/L范围内线性关系良好（r2>0999）。人参皂苷Rg1、Re和Rb1的定量下限（S/N=10）分别为1769、1394、114 mg/L。3个不同浓度的加标回收率为891%~107%，相对标准偏差（RSD，n=3）为06%~41%。该方法具有操作简便快捷、检测灵敏度高等特点。 |
| 中文关键词:人参皂苷 高效液相色谱 中药渣 |
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| Determination of Ginsenosides Rg1,Re and Rb1 in Chinese Herbal Residues by HPLC |
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| Abstract:A fast and sensitive reversed high performance liquid chromatographic（HPLC） method was developed for the simultaneous detection of three ginsenosides,ie.Rg1,Re and Rb1 in Chinese herbal residues.Effects of solvent,temperature,solvent matrix ratio and time on extraction efficiency were investigated.The sample was ultrasonically extracted with 10 times liquid of 70% methanol at 40 ℃ for 05 h.Chromatographic separation was performed on a Zorbax SB-C18（46 mm×150 mm,5 μm） column by gradient elution using water-acetonitrile as mobile phase at a flow rate of 10 mL/min.The injection volume was 20 μL,the column temperature was 20 ℃ and the detection wavelength was 203 nm.External calibration of peak area versus concentration was used for the quantitation.There were excellent linearities for ginsenosides Rg1，Re and Rb1 in the ranges of 1874-25761,3748-46854 and 388-9693 mg/L,respectively，with their correlation coefficients all above 0999.The limits of quantitation（LOQ,S/N=10） for Rg1,Re and Rb1 were 1769,1394 and 114 mg/L,respectively.The average recoveries at three spiked levels were in the range of 891%-107%,with relative standard deviations（RSD,n=3） of 06%-41%.The method was applied in the detection of ginsenosides Rg1，Re and Rg1 in Chinese herbal samples with simplicity,rapidness and high sensitivity. |
| Key Words:ginsenosides high performance liquid chromatography（HPLC） Chinese herbal residues |
| 引用本文：陈闻,许丽卫,耿梅梅,张丽萍,袁红朝,李春勇,彭灿,王久荣,孔祥峰.HPLC测定中药渣中人参皂苷Rg1、Re与Rb1含量[J].分析测试学报,2018,37(8):935-939. |
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