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UPLC法测定不同加工方式鹿茸中的生物胺成分
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作者单位
王燕华,孙印石,王玉方,陈宝,霍晓慧,刘畅,张磊 1.中国农业科学院特产研究所2.吉林农业大学中药材学院 
基金项目:吉林省科技发展计划项目(20170201034YY,20170309002YY,20170311027YY);中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-ISAPS)
中文摘要:首次应用超高效液相色谱法(UPLC)同时测定了鹿茸中10种生物胺组分的含量,比较了不同加工方式的鹿茸中生物胺的差异。鹿茸样品中的生物胺用0.4 mol/L高氯酸浸提,10 g/L丹磺酰氯衍生化,UPLC法定量分析。色谱柱为ACQUITY UPLC@BEH C18(2.1 mm×100 mm,1.7 μm),流动相为乙腈-水,柱温为35 ℃,流速为0.4 mL/min,检测波长为217 nm。结果显示,10种生物胺在一定浓度范围内呈良好线性关系,其相关系数为0.997 8~0.999 9,检出限为10.87~19.63 μg/L,回收率为71.6%~101%。煮炸茸和冻干茸蜡片、粉片、纱片、骨片4个部位的生物胺总量依次为312.33、176.88、105.31、55.674 mg/kg和291.77、152.85、114.49、74.73 mg/kg;排血茸蜡片、粉片、纱片、骨片4个部位的生物胺总量分别为357.07、226.26、125.18、77.74 mg/kg,带血茸的分别为343.42、216.72、125.15、76.16 mg/kg。就生物胺总量而言,冻干茸高于煮炸茸,排血茸高于带血茸,且按蜡片、粉片、纱片、骨片部位依次减少,不同部位之间差异显著(P<0.05)。
中文关键词:鹿茸  生物胺  加工方式  部位  比较  超高效液相色谱法(UPLC)
 
Simultaneous Determination of Biogenic Amine in Cervi Cornu Pantotrichum Processed with Different Methods by UPLC
Abstract:A method of ultra high performance liquid chromatography(UPLC) was developed for the simultaneous determination of 10 biogenic amine components in Cervi Curnu Pantotrichum(CCP),and the differences among the biogenic amines in Cervi Curnu Pantotrichum(CCP) processed with different methods were compared.Biogenic amines in the CCP samples were extracted with 0.4 mol/L perchloric acid,derivatized with dansyl chloride(DNCSI),then determinated by UPLC.The optimal conditions were follows:chromatographic column:ACQUITY UPLC@BEH C18(2.1 mm×100 mm,1.7 μm),mobile phase:acetonitrile-water,column temperature:35 ℃,flow rate:0.4 mL/min,detection wavelength:217 nm.The calibration curves for 10 biogenic amines were linear in a certain concentration with their correlation coefficients of 0.997 8-0.999 9 and the detection limits of 10.87-19.63 μg/L.The recoveries ranged from 71.6% to 101%.The total biogenic amines in wax slice,powder slice,gauze slice and bone slice for the boiled CCP were 312.33,176.88,105.31 and 55.674 mg/kg,respectively,and that for the freeze-dried CCP were 291.77,152.85,114.49 and 74.73 mg/kg,respectively.Likewise,those in these 4 sections for the blood-drained CCP and the blood-filled CCP were 357.07,226.26,125.18 and 77.74 mg/kg,and 343.42,216.72,125.15 and 76.16 mg/kg,respectively.As a whole,the total biogenic amines in freeze-dried CCP were higher than that in boiled CCP,while those in blood-drained CCP were higher than that in blood-filled CCP.The total biogenic amines in CCP with 4 processing methods showed a trend of decline from wax slice downward to bone slice,and the differences in different sections were all significant(P<0.05).
Key Words:Cervi Curnu Pantotrichum  biogenic amines  processing methods  sections  comparative analysis  ultra high performance liquid chromatography(UPLC)
引用本文:王燕华,孙印石,王玉方,陈宝,霍晓慧,刘畅,张磊.UPLC法测定不同加工方式鹿茸中的生物胺成分[J].分析测试学报,2018,37(9):995-1001.
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