| 石墨烯-壳聚糖修饰电极构建石杉碱甲免疫传感器 |
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| 基金项目:国家自然基金项目（81202914, 81703695）；福建省科技厅引导性项目（2016Y0056）；福建省教育厅科研项目（JAT160238） |
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| 中文摘要:通过将石杉碱甲抗体（anti-HupA）固定到由还原态氧化石墨烯/壳聚糖（rGO/CS）复合膜修饰的玻碳电极表面，制备高灵敏度的电化学免疫传感器，利用石杉碱甲抗原抗体特异性反应可阻断［Fe（CN）6］3-/4-氧化还原反应体系电子转移的特点，建立了检测石杉碱甲（HupA）的电化学免疫传感器分析方法。采用循环伏安法（CV）、交流阻抗法（EIS）及差示脉冲伏安法（DPV）研究HupA在［Fe（CN）6］3-/4-氧化还原体系的电化学行为，利用DPV法测定HupA浓度。在优化条件下，15 mmol/L K3［Fe（CN）6］+0.1 mol/L KCl+0.1 mol/L PBS（pH 6.5）测试体系中，HupA浓度的对数与峰电流差值在1.0×10-13~1.0×10-10 mol/L范围内呈良好的线性关系，相关系数（r）为 1.000 0，检出限为3.0×10-14 mol/L，回收率为99.4%~102%。该方法具有特异性强、灵敏度高、操作简单、绿色环保等特点，可用于中药材中石杉碱甲浓度的快速检测。 |
| 中文关键词:石墨烯 壳聚糖 石杉碱甲 电化学免疫传感器 差示脉冲伏安法 |
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| Construction of Huperzine A Immunosensor with Graphene-Chitosan Modified Electrode |
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| Abstract:A high-sensitive electrochemical immunosensor was prepared by immobilizing anti-HupA monoclonal antibody onto the glassy carbon electrode modified with a reduced graphene oxide/chitosan composite membrane.And an electrochemical immunosensor for the detection of huperzine A（HupA）was established as the antigen-anibody reaction could block the electron transfer of［Fe（CN）6］3-/4- redox system.The electrochemical behavior of HupA in［Fe（CN）6］3-/4- redox system was investigated by cyclic voltammetry（CV），electrochemical impedance spectroscopy（EIS）and differential pulse voltammetry（DPV）.Under the optimized conditions, there existed a good linearity between HupA concentration logarithm and the difference of peak current in the assay system of 15 mmol/L K3Fe（CN）6+0.1 mol/L KCl+0.1 mol/L PBS（pH 6.5） in range of 1.0×10-13-1.0×10-10 mol/L with a correlation coefficient（r）of 1.000 0.The detection limit was 3.0×10-14 mol/L, and the recoveries were in the range of 99.4%-102%.With the characteristics of strong specificity, high sensitivity, simple operation and environmental protection, this method could be used for the rapid detection of huperzine A in natural herbs. |
| Key Words:graphene chitosan huperzine A electrochemical immunosensor differential pulse voltammetry（DPV） |
| 引用本文：陈媛媛,高璇,刘媛媛,尤金坤，张红艳,余宇燕.石墨烯-壳聚糖修饰电极构建石杉碱甲免疫传感器[J].分析测试学报,2018,37(9):1014-1019. |
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