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四磺化酞菁锌与白蛋白复合物的制备、光谱性质与光动力活性研究
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作者单位
张丽娟,郑碧远,李洪才,黄剑东 1.福建生物工程职业技术学院药学系2.福州大学化学学院 
基金项目:国家自然科学基金项目(21473033)
中文摘要:通过光谱法研究了四磺化酞菁锌(ZnPcPS4)与白蛋白(BSA和HSA)的相互作用。结果表明,白蛋白对ZnPcPS4的存在状态影响显著,两者存在较强的相互作用,结合常数为106数量级。结合位点竞争性实验表明,其相互作用的结合位点主要位于牛血清白蛋白的亚域ⅠB。进一步制备了ZnPcPS4-BSA和ZnPcPS4-HSA复合物,复合物中ZnPcPS4和白蛋白的摩尔比约为1∶1。光谱研究表明,复合物展现出比游离ZnPcPS4治疗更明显的单体特征吸收峰,且Q带最大吸收波长红移(从692 nm红移至696 nm),这有利于光动力治疗作用。光动力抗癌活性测试表明,ZnPcPS4-BSA具有较高的光动力抗癌活性,其IC50值仅为1.68 μmol/L,活性明显高于其对应的游离ZnPcPS4(IC50=2.73 μmol/L),可归因于复合物具有更高的癌细胞摄取率。
中文关键词:磺化酞菁  白蛋白  光动力疗法  光谱性质
 
Preparation,Spectroscopic Properties and Photodynamic Activity of Conjugates of Albumin and Tetrasulfonated Zinc Phthalocyanine
Abstract:The interaction between tetrasulfonated zinc phthalocyanine(ZnPcPS4) and albumin(BSA and HSA) was studied by fluorescence spectroscopy.The results showed that albumin had a significant effect on the existing state of ZnPcPS4,and there was a strong interaction between ZnPcPS4 and albumin with a binding constant about 106 orders of magnitude.The competitive binding experiment showed that the binding site for the interaction was mainly located at subdomain IB of BSA.The ZnPcPS4-BSA and ZnPcPS4-HSA complexes were further prepared by a molar ratio of phthalocyanine to albumin about 1∶1.Spectroscopic studies showed that the complexes exhibited a more prominent monomer absorption peak than free phthalocyanine,and the Q-band maximum had a red shift from 692 nm to 696 nm,which was beneficial to photodynamic therapy.Photodynamic anticancer activity test showed that ZnPcPS4-BSA had a high photodynamic anticancer activity with an IC50 value as low as 1.68 μmol/L,which was significantly higher than that of the corresponding free phthalocyanine ZnPcPS4(IC50=2.73 μmol/L).This could be attributed to the fact that the complexes have a higher cellular uptake toward cancer.
Key Words:sulfonated phthalocyanine  albumin  photodynamic therapy  spectroscopic properties
引用本文:张丽娟,郑碧远,李洪才,黄剑东.四磺化酞菁锌与白蛋白复合物的制备、光谱性质与光动力活性研究[J].分析测试学报,2018,37(9):1020-1026.
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