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水介质中二甲基姜黄素脂质体对金属离子的荧光识别
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作者单位
李正义,邵丹迪,殷乐,肖唐鑫,孙小强 常州大学石油化工学院 
基金项目:国家自然科学基金资助项目(21572026);江苏省先进催化与绿色制造协同创新中心项目(ACGM2016-06-05)
中文摘要:通过薄膜分散法制备二甲基姜黄素(ASC-J9)脂质体,其粒径分布均匀且在水中的分散效果好,平均粒径为145.7 nm,分散系数为0.361。将ASC-J9脂质体作为水溶性的荧光探针,通过荧光猝灭法可选择性识别Fe3+、Fe2+及Cu2+。经过条件筛选得到最佳荧光测试条件为:ASC-J9脂质体浓度为5.0×10-5mol/L,平衡时间5 min,测试温度25 ℃。由Jobs 曲线和荧光滴定结果判断该探针与Fe3+和Cu2+的络合比均为1∶1,与Fe2+的络合比为2∶1;Stern-Volmer方程判断该荧光猝灭类型为静态猝灭,结合常数分别为KFe(Ⅲ)=9.63×104 L/mol,KFe(Ⅱ)=3.65×105 L/mol,KCu(Ⅱ)=2.32×105 L/mol;该识别体系检测快速、灵敏度高,对Fe3+、Fe2+、Cu2+的线性范围分别为:5.0×10-7~3.0×10-5、5.0×10-7~1.75×10-5、5.0×10-7~2.5×10-5 mol/L,检出限分别为6.41×10-7、3.28×10-7、5.08×10-7mol/L。
中文关键词:二甲基姜黄素(ASC-J9)  脂质体  荧光探针  金属离子  
 
Fluorescent Recognition of Metal Ions in Water Using Dimethyl Curcumin Liposome
Abstract:A water-soluble molecular fluorescence probe,dimethyl curcumin(ASC-J9)liposome was developed for the recognition of Fe3+,Fe2+ and Cu2+ through fluorescence quenching.The ASC-J9 liposome was prepared by thin-film dispersion,which had a uniform particle size distribution with a particle size of 145.7 nm and a polymer dispersity index(PDI)of 0.361.The optimal fluorescence conditions obtained by conditional screening were as follows:ASC-J9 liposome concentration:5.0×10-5 mol/L,balance time:5 min,temperature:25 ℃.The binding modes of ASC-J9 liposome with Fe3+,Cu2+ and Fe2+ were achieved by Jobs plot and fluorescence titration.Results showed that the binding ratios for the probe to Fe3+ and Cu2+ were both 1∶1,while that for the probe to Fe2+ was 2∶1.The fluorescence quenching type was determined by Stern-Volmer plot as static quenching,and the association constants of KFe(Ⅲ),KFe(Ⅱ)and KCu(Ⅱ)were 9.63×104 L/mol,3.65×105 L/mol and 2.32×105 L/mol,respectively.With the advantages of rapid detection and high sensitivity,this method was applied in the identification of Fe3+,Fe2+ and Cu2+ ions in water samples.The calibration curves for Fe3+,Fe2+ and Cu2+ were 5.0×10-7 -3.0×10-5 mol/L,5.0×10-7 -1.75×10-5 mol/L and 5.0×10-7 -2.5×10-5 mol/L,with their limits of detection of 6.41×10-7 mol/L,3.28×10-7 mol/L and 5.08×10-7 mol/L,respectively.
Key Words:dimethyl curcumin(ASC-J9)  liposome  fluorescence probe  metal ion  water
引用本文:李正义,邵丹迪,殷乐,肖唐鑫,孙小强.水介质中二甲基姜黄素脂质体对金属离子的荧光识别[J].分析测试学报,2018,37(9):1046-1050.
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