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基于生物芯片的核酸凝胶电泳仪
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作者单位
游庆祥,陶春先,张大伟,庄松林,李振庆,山口佳则 1.上海理工大学光电信息与计算机工程学院上海市现代光学系统重点实验室
2.常州工学院计算机信息工程学院
3.华东理工大学物理系
4.大阪大学应用物理系 
基金项目:国家自然科学基金(21205078);高等学校博士学科点专项科研基金博导类资助课题(20123120110002)
中文摘要:为克服传统平板凝胶电泳法操作步骤繁琐,实验时间长,以及采用紫外光源可能对人体造成伤害等缺陷,该文研制了一种基于电泳芯片的快速凝胶电泳仪,仅需DNA点样于电泳芯片后,将电泳芯片置于该电泳仪便可实现DNA样品快速分离及实时在线成像检测。以20、50、100 bp DNA ladder为检测对象,在仪器内对其分离效果进行验证及优化,其最佳电泳条件为电压100 V/cm,琼脂糖质量分数分别为2.5%、1.0%、1.4%。结果表明在14 min内可以实现3种DNA样品的高效分离,DNA迁移距离与分子量的相关系数均高于0.9,且该仪器具有较高的稳定性。
中文关键词:电泳芯片  DNA  电泳仪
 
A Nucleic Acid Gel Electrophoresis System Based on Biochip
Abstract:As traditional slab gel electrophoresis has some disadvantages such as tedious operation steps,long experimental time and possible damage to human health from ultraviolet light,a rapid gel electrophoresis system was developed based on electrophoresis biochip,which could realize the separation and imaging of DNA by adding the DNA sample into the biochip.Taking the 20,50 and 100 bp DNA ladder as target compounds,the DNA fragments were separated in the gel electrophoresis system,and the optimal electrophoretic parameters were as follows:the electric field strength:100 V/cm,the agarose mass concentration:1%.Results showed that all the DNA samples could be efficiently resolved in 14 min with the correlation coefficient between migration distance and the DNA size higher than 0.91.Besides,the instrument could run with a good stability.
Key Words:electrophoresis biochip  DNA  electrophoresis system
引用本文:游庆祥,陶春先,张大伟,庄松林,李振庆,山口佳则.基于生物芯片的核酸凝胶电泳仪[J].分析测试学报,2018,37(9):1092-1095.
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全文下载次数: 51
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