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CRISPR/Cas技术在核酸检测中的应用进展
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作者单位
史铠,雷春阳,聂舟 湖南大学化学生物传感与计量学国家重点实验室 
基金项目:国家自然科学基金资助项目(21725503)
中文摘要:CRISPR/Cas是大多数细菌及古细菌基于RNA的后天免疫系统。由CRISPR/Cas系统改造而成的CRISPR/Cas技术已成为一种强大的基因编辑工具,广泛应用于基因功能研究和基因修饰与治疗。除了作为基因编辑工具,Ⅱ类Cas蛋白具有的“附属切割”特性,已被开发成一种快速、低成本且高灵敏的核酸检测工具,在核酸分子诊断领域具有重要的应用潜力。该文总结了3种Ⅱ类Cas蛋白在核酸检测领域的代表性研究进展,并对CRISPR/Cas系统在该领域的应用前景进行了展望。
中文关键词:CRISPR  Cas蛋白  特异性识别  附属切割  核酸检测
 
Application Progress of CRISPR/Cas in Nucleic Acid Detection
Abstract:Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)is an adaptive immune defense system based on RNA for most bacteria and archaea.CRISPR/Cas technology modified from CRISPR/Cas system has become a strong genome editing tool,which has been widely applied in gene function analysis,gene modification and therapy.Other than being a strong genome editing tool,the “collateral cleavage” of type Ⅱ Cas has been employed as a rapid,inexpensive,and sensitive nucleic acid detection tool,exhibiting a great promise in nucleic acid molecular diagnosis.The representative research progresses in nucleic acid detection based on the type Ⅱ Cas are reviewed in this article,and an outlook on the application of CRISPR/Cas system in molecular diagnosis is proposed.
Key Words:CRISPR  Cas proteins  specific recognition  collateral cleavage  nucleic acid detection
引用本文:史铠,雷春阳,聂舟.CRISPR/Cas技术在核酸检测中的应用进展[J].分析测试学报,2018,37(10):1217-1220.
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