| 温敏型铜银荧光纳米簇的绿色制备及细菌成像研究 |
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| 基金项目:国家自然科学基金（51702128）；江苏省自然科学基金（BK20170210） |
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| 中文摘要:在温和条件下以D 青霉胺为配体，通过一步法制备出水溶性的具有红色荧光的铜银纳米簇（Cu-AgNCs），并对其形貌、结构、荧光性能等进行了表征。结果表明，制备得到的纳米簇的量子产率可达96%；在279~355 K温度范围内，该纳米簇的荧光强度与温度之间呈良好的线性关系，荧光强度随着温度的增加而减弱。此外，细胞毒性噻唑蓝（MTT）实验结果表明，合成的Cu-AgNCs显示出低毒性和较好的生物相容性，它们可以渗透大肠杆菌进行细胞成像。采用共聚焦荧光成像方法在293~313 K的生理温度范围内进行细菌体内温度测量，结果表明，制备的Cu-AgNCs有望作为光致发光温度计和生物传感器应用于荧光成像等领域。 |
| 中文关键词:铜银纳米簇 温度响应 大肠杆菌 荧光成像 |
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| Green Preparation and Bacteria Imaging of Thermo sensitive Cu-Ag Fluorescent Nanoclusters |
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| Abstract:In this paper,the water soluble copper-silver nanoclusters（Cu-AgNCs）with red fluorescence were prepared by one step method with D penicillamine as ligand under mild conditions.The morphology,structure and fluorescence properties were characterized.Result showed that the quantum yield of the prepared nanoclusters reached up to 96%,and there was a good linear relationship between fluorescence intensity and temperature of the nanocluster in the range of 279-355 K.With the increase of temperature,the fluorescence intensity decreased.In addition,the results of MTT experiments showed that as the synthesized Cu-AgNCs exhibited a low toxicity and a good biocompatibility,they could permeate into E.coli cells,and enable measurements over the physiological temperature range of 293-313 K with the use of the confocal fluorescence imaging method.The results showed that the prepared Cu-AgNCs were expectable to use as a photoluminescence thermometer and biosensor in fluorescence imaging and other fields. |
| Key Words:copper silver nanoclusters temperature response E.coli fluorescence imaging |
| 引用本文：陆可钰，孔令灿，潘红阳，朱松.温敏型铜银荧光纳米簇的绿色制备及细菌成像研究[J].分析测试学报,2018,37(10):1258-1263. |
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