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温敏型铜银荧光纳米簇的绿色制备及细菌成像研究
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陆可钰,孔令灿,潘红阳,朱松 1.江南大学食品科学与技术国家重点实验室江南大学分析测试中心2.无锡市疾病预防控制中心 
基金项目:国家自然科学基金(51702128);江苏省自然科学基金(BK20170210)
中文摘要:在温和条件下以D 青霉胺为配体,通过一步法制备出水溶性的具有红色荧光的铜银纳米簇(Cu-AgNCs),并对其形貌、结构、荧光性能等进行了表征。结果表明,制备得到的纳米簇的量子产率可达96%;在279~355 K温度范围内,该纳米簇的荧光强度与温度之间呈良好的线性关系,荧光强度随着温度的增加而减弱。此外,细胞毒性噻唑蓝(MTT)实验结果表明,合成的Cu-AgNCs显示出低毒性和较好的生物相容性,它们可以渗透大肠杆菌进行细胞成像。采用共聚焦荧光成像方法在293~313 K的生理温度范围内进行细菌体内温度测量,结果表明,制备的Cu-AgNCs有望作为光致发光温度计和生物传感器应用于荧光成像等领域。
中文关键词:铜银纳米簇  温度响应  大肠杆菌  荧光成像
 
Green Preparation and Bacteria Imaging of Thermo sensitive Cu-Ag Fluorescent Nanoclusters
Abstract:In this paper,the water soluble copper-silver nanoclusters(Cu-AgNCs)with red fluorescence were prepared by one step method with D penicillamine as ligand under mild conditions.The morphology,structure and fluorescence properties were characterized.Result showed that the quantum yield of the prepared nanoclusters reached up to 96%,and there was a good linear relationship between fluorescence intensity and temperature of the nanocluster in the range of 279-355 K.With the increase of temperature,the fluorescence intensity decreased.In addition,the results of MTT experiments showed that as the synthesized Cu-AgNCs exhibited a low toxicity and a good biocompatibility,they could permeate into E.coli cells,and enable measurements over the physiological temperature range of 293-313 K with the use of the confocal fluorescence imaging method.The results showed that the prepared Cu-AgNCs were expectable to use as a photoluminescence thermometer and biosensor in fluorescence imaging and other fields.
Key Words:copper silver nanoclusters  temperature response  E.coli  fluorescence imaging
引用本文:陆可钰,孔令灿,潘红阳,朱松.温敏型铜银荧光纳米簇的绿色制备及细菌成像研究[J].分析测试学报,2018,37(10):1258-1263.
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