| 线性离子阱飞行时间质谱仪的研制及性能表征 |
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| 基金项目:2016广州市产学研协同创新重大专项产业技术研究（201604010065）；重大科学仪器设备开发专项（2017YFF010600005） |
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| 中文摘要:基于飞行时间质谱技术、线性离子阱技术、大气压电离源等核心技术，自制了一款台式高分辨线性离子阱飞行时间联用质谱仪器（LIT-TOF MS）。以电喷雾离子源对仪器性能进行表征：LIT-TOF MS的质量分辨率超过12 000（利血平m/z 609），质量范围达到33~1 922 amu，灵敏度为1 μg/L；能够实现MS3的三级质谱分析效果。对氨基酸进行了定性及定量的初步应用分析，结果表明：LIT-TOF MS可为代谢组学研究中实时、在线、高通量测定生物复杂样品中氨基酸的含量及其变化提供一定依据。该仪器能与多种常压电离技术联用，有望用于药品、环境、食品等领域。 |
| 中文关键词:电喷雾离子源 线性离子阱 飞行时间质谱仪 联用质谱 |
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| Development and Performance Characterization of a Linear Ion Trap Time of Flight Mass Spectrometer |
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| Abstract:A high resolution hybrid linear ion trap time of flight mass spectrometer（LIT-TOF MS） was constructed，which is based on the time of flight mass spectrometry，linear ion trap，atmospheric pressure ionization source and other core technologies.The performance of the instrument was characterized with an electric spraying ion source.The resolution of LIT-TOF MS was over 12 000（Reserpine，m/z 609），the mass range was 33-1 922 amu，and the sensitivity was 1 μg/L.It was also demonstrated that MS3 could be readily performed in the LIT.The qualitative and quantitative analyses on amino acids were carried out by the LIT-TOF MS，and results showed that it could be used in the real time，on line and high throughput determination of contents and variations of amino acids in biological complex samples.Besides，the instrument，combined with a variety of atmospheric pressure ionization techniques，could be applicable for determination in fields of medicine，environment and foods，etc. |
| Key Words:electrospray ion source linear ion trap time of flight mass spectrometer hybrid mass spectrometer |
| 引用本文：黄晓,田琴琴,范荣荣,李辉,黄保,邹星,朱辉,黄正旭.线性离子阱飞行时间质谱仪的研制及性能表征[J].分析测试学报,2018,37(11):1322-1327. |
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