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UHPLC-ESI-MS/MS法测定动物源食物提取物中肌肽与鹅肌肽的含量
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作者单位
王雨晴,陈亮,贾福怀,郑锌,李月琪,马勇,张海欣,王憬,谷瑞增 1.中国食品发酵工业研究院有限公司北京市蛋白功能肽工程技术研究中心2.宁波御坊堂生物科技有限公司浙江宁波3150123.岛津企业管理(中国)有限公司 
基金项目:十三五国家重点研发计划(2016YFD0400604);国家自然科学基金(31671963)
中文摘要:建立了一种同时测定动物源提取物中肌肽(Carnosine)和鹅肌肽(Anserine)含量的超高效液相色谱-电喷雾串联三重四极杆质谱(UHPLC-ESI-MS/MS)分析方法。采用超高效液相分离系统,借助InertsilAmide HP色谱柱(2.1 mm×100 mm,3 μm) 以01%乙酸水溶液-乙腈为流动相,梯度洗脱分离,利用峰面积外标法定量。经过优化,肌肽和鹅肌肽在1.95~500 μg/L质量浓度范围内与峰面积呈良好的线性关系,相关系数(r)均大于0.998。肌肽和鹅肌肽的检出限分别为0.12、0.47 μg/L。以低浓度低聚肽原料为基质,在低、中、高3个加标浓度下,肌肽的回收率为99.3%~109%,相对标准偏差(RSD,n=6)为0.98%~1.1%;鹅肌肽的回收率为100%~113%,RSD为1.0%~1.1%。该法前处理简单、快速,重现性好,准确度高,适用于动物源食物提取物中肌肽和鹅肌肽的同时快速检测。
中文关键词:超高效液相色谱-电喷雾串联质谱  肌肽  鹅肌肽  动物源食物提取物
 
Determination of Carnosine and Anserine in Animal derived Food Extracts by UHPLC-ESI-MS/MS
Abstract:A method was established for the simultaneous determination of carnosine and anserine in animal derived food extracts by ultrahigh performance liquid chromatography-electrospray ionization triple quadrupole tandem mass spectrometry.The separation on the analytes was performed by an ultrahigh performance liquid separation system with an Inertsil Amide HP(2.1 mm×100 mm,3 μm) column using 0.1% acetic acid and acetonitrile as mobile phases by gradient elution,and the external standard method of peak area was used for quantitation.There existed good linear relationships for the peak areas of carnosine and anserine in the concentration range of 1.95-500 μg/L,with their correlation coefficients(r) larger than 0998.The detection limits for carnosine and anserine were 0.12 μg/L and 0.47 μg/L,respectively.The low concentration oligopeptide raw material was used as the substrate,and the quality control of 2,20,and 200 μg/L was spiked.The recoveries for carnosine and anserine at three spiked levels of 2,20 and 200 μg/L were in the ranges of 993%-109% and 100%-113%,with their relative standard deviations(RSD,n=6) of 0.98%-1.1% and 1.0%-1.1%,respectively.With the advantages of simple pretreatment,rapidness,good reproducibility and high precision,the method is applicable for the simultaneous detection of carnosine and anserine in animal derived food extracts.
Key Words:ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-ESI-MS/MS)  carnosine  anserine  animal derived food extract
引用本文:王雨晴,陈亮,贾福怀,郑锌,李月琪,马勇,张海欣,王憬,谷瑞增.UHPLC-ESI-MS/MS法测定动物源食物提取物中肌肽与鹅肌肽的含量[J].分析测试学报,2018,37(11):1328-1333.
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