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红枣中4种链格孢菌毒素的检测及风险评估
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作者单位
程家兴,黎明阳,杨雨希,杨茜,袁涤,杨颉,王毅聪,张锐利,杜振霞 1.北京化工大学理学院2.塔里木大学生命科学学院 
基金项目:中央高校基本科研业务专项资金(ZZ1706);新疆红枣链格孢毒素污染发生规律及其调控机理研究(U1703234)
中文摘要:建立了超高效液相色谱-三重四极杆质谱(UPLC-MS/MS)快速检测红枣中链格孢菌代谢产生的细交链格孢菌酮酸(TEA)、链格孢酚(AOH)、交链格孢酚单甲醚(AME)和腾毒素(TEN)4种链格孢菌毒素的方法。采用2.0%甲酸-乙腈溶液超声提取,辅以0.2 g NaCl盐析,使用BEH C18色谱柱在6 min内快速分离毒素,并采用多反应监测模式(MRM)检测红枣中的4种链格孢菌毒素。除了链格孢酚的线性范围为1.0~100 μg/L外,其他3种毒素的线性范围均为10~1 000 μg/L,线性关系(r2)均大于0.990,检出限为0.5~12.4 μg/kg。4种毒素在空白基质中的加标回收率为92.8%~116%,相对标准偏差为0.5%~11.2%,可满足红枣中链格孢菌毒素的检测要求。运用毒理学软件理论计算得到成年人对4种毒素的允许每日暴露量(PDE)为19.30~25.65 μg/d。
中文关键词:超高效液相色谱-串联质谱(UPLC-MS/MS)  链格孢菌毒素  红枣  风险评估
 
Determination and Risk Assessment of Four Alternaria Mycotoxins in Jujube
Abstract:A noval method was established for the detection of four alternaria mycotoxins,iealternariol,alternariol monomethyl ether,tenuazonic acid and tentoxin in jujube by ultrahigh performance liquid chromatography-tandem mass spectrometry.The sample was extracted with 2.0% formic acid-acetonitrile by sonication and auxiliarily salted out with 0.2 g NaCl.Four alternaria mycotoxins in jujube were separated on a BEH C18 column in 6 min,and dectected in multiple reaction monitoring mode(MRM).The calibration curves for 4 alternaria mycotoxins showed good linearities in the ranges of 1.0-100 μg/L for alternariol and 10-1 000 μg/L for the other three toxins,with their correlation coefficients larger than 0.990 and the detection limits of 0.5-12.4 μg/kg.The spiked recoveries for 4 toxins in blank matrix were between 92.8% and 116% with the relative standard deviations of 0.5%-11.2%,which could meet the requirements for detection of alternaria mycotoxin in jujube.Moreover,the permitted daily exposures(PDE) for adults against 4 types of toxins were obtained to be 19.30-25.65 μg/d based on the calculation of the toxicological software theory.
Key Words:ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)  alternaria mycotoxins  jujube  risk assessment
引用本文:程家兴,黎明阳,杨雨希,杨茜,袁涤,杨颉,王毅聪,张锐利,杜振霞.红枣中4种链格孢菌毒素的检测及风险评估[J].分析测试学报,2018,37(11):1334-1338.
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