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分子印迹固相萃取/毛细管电泳法检测牛奶中头孢噻肟残留
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作者单位
陈玎玎,何东旭,祁克宗,陆翠珍 1.安徽农业大学生物技术中心2.安徽农业大学动物科技学院 
基金项目:国家“十一五”科技支撑项目子项目(2006BAK02A08-4);安徽高校省级自然科学研究项目(KJ2011B065)
中文摘要:采用分子印迹技术,以头孢噻肟(CTX)为模板分子,α-甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂合成了头孢噻肟分子印迹聚合物(CTX-MIP)。以该分子印迹聚合物为固相萃取柱填料,毛细管电泳进行检测,建立了分子印迹固相萃取/毛细管电泳检测牛奶中头孢噻肟残留的方法。结果表明,CTX-MIP对CTX具有较高的选择性,萃取效果良好。该方法在CTX为5~100 mg/L范围内呈良好线性,相关系数(r2)为0.999 4;3种不同加标水平(10、50、100 mg/L)的回收率分别为78%、84%、86%,相对标准偏差(RSD)为3.5%~4.5%,检出限(LOD,S/N≥3)为98.52 μg/L,定量下限(LOQ,S/N≥10)为329.0 μg/L,符合兽药残留分析的要求。
中文关键词:分子印迹固相萃取  毛细管电泳  头孢噻肟  牛奶
 
Determination of Cefotaxime Residue in Milk by Molecular Imprinted Solid Phase Extraction/Capillary Electrophoresis
Abstract:A molecularly imprinted polymer for cefotaxime(CTX-MIP) was synthesized by the molecular imprinting technique using cefotaxime(CTX) as template molecule,α-methacrylic acid(MAA) as functional monomer,ethylene glycol dimethacrylate(EGDMA) as cross linking agent.By using the cefotaxime molecularly imprinted polymer as sorbent in solid-phase extraction column,a molecularly imprinted solid phase extraction-capillary electrophoresis(MISPE-HPCE) method was developed for the determination of cefotaxime residue in milk.The results showed that cefotaxime imprinted polymer exhibited a higher selectivity to cefotaxime compared with that of the non imprinted polymer.The linear range of cefotaxime was in the range of 0.5-100 mg/L,and the correlation coefficient was 0.999 4.The recoveries at three spiked concentration levels of 10,50,100 mg/L were 78%,84% and 86%,respectively,with RSDs of 3.5%-4.5%.The limit of detection(LOD,S/N≥3) and the limit of quantitation(LOQ,S/N≥10) were 98.52 μg/L and 329.0 μg/L,respectively.The method could meet the requirements for veterinary drug residue analysis.
Key Words:molecularly imprinted solid phase extraction  capillary electrophoresis  cefotaxime  milk
引用本文:陈玎玎,何东旭,祁克宗,陆翠珍.分子印迹固相萃取/毛细管电泳法检测牛奶中头孢噻肟残留[J].分析测试学报,2012,31(10):1334-1338.
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