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选择性富集分离虎杖中白藜芦醇苷的分子印迹聚合物的制备及分子识别性能研究
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作者单位
向海艳,范银洲,李曼,卓冬梅,谢扬 1.南方医科大学药学院
2.南方医科大学中医药学院 
基金项目:广东省教育部产学研资助项目(2009B090300216)
中文摘要:以白藜芦醇苷(POL)为模板分子,分别以丙烯酰胺(AM)、4-乙烯基吡啶(4-VP)、甲基丙烯酸羟乙酯(HEMA)、甲基丙烯酸(MAA)为功能单体,二甲基丙烯酸乙二醇酯(EGDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,采用本体聚合法制备白藜芦醇苷分子印迹聚合物。采用静态平衡结合实验研究了印迹聚合物对模板分子及不同底物的识别性能。结果表明,以丙烯酰胺为功能单体的印迹聚合物(MIP1)对模板分子的识别性能最好,其次是以4-VP为功能单体的聚合物(MIP2),以HEMA为功能单体的聚合物(MIP3)以及以MAA为功能单体的聚合物(MIP4)的分子识别性能较差。表明功能单体与模板分子之间相互作用的强弱对MIP的识别能力有较大的影响。静态平衡结合法以及Scatchard分析法表明,MIP1对模板分子呈现较好的结合能力和选择性,该印迹聚合物中形成了2类不同的结合位点,离解常数分别为7.43×10-5、3.70×10-3 mol/L。将MIP1用于虎杖提取物中POL的固相萃取分离,效果良好。
中文关键词:分子印迹聚合物  白藜芦醇苷  识别性能  虎杖提取物  固相萃取
 
Synthesis and Study of Molecularly Imprinted Polymers for the Specific Extraction of Polydatin from Polygonum Cuspidatum Sieb.et Zucc.Extracts
Abstract:Molecularly imprinted polymers(MIPs) were synthesized by bulk polymerization using polydatin(POL) as template molecule,acrylamide(AM),4-vinylpyridine(4-VP),2-hydroxyethylmetacrylate(HEMA) or methacrylic acid(MAA) as functional monomer,and ethyleneglycol dimethacrylate(EGDMA) as crosslinking agent,initiating thermally with azobisisobutyronitrile(AIBN).The binding and selectivity of MIPs to POL and different substrates were evaluated by the static batch method.MIP1,which was synthesized with AM as functional monomer,had the best recoginition property to the template,followed by MIP2 sythesized with 4-VP as functional monomer,MIP3 synthesized with HEMA as functional monomer and MIP4 synthesized with MAA as functional monomer.The results indciated that the interactions between the template and the functional monomer in the solvent influenced the recognition property of MIPs.Static equilibrium binding experiments and the scatchard analysis showed that MIP1 existed good selectivity and recognition properties to the template POL.Two kinds of binding sites were produced in the MIP1 matrix and their dissociation constants were calculated to be 7.43×10-5 mol/L and 3.70×10-3 mol/L,respectively.Compared with non-imprinted polymer(NIP1),the imprinted polymer(MIP1) exhibits a higher affinity for POL among the mixed substrates.The MIP1 was applied in the specific extraction of POL from Polygonum cuspidatum Sieb.et Zucc.extracts with satisfactory results.
Key Words:molecularly imprinted polymers(MIPs)  polydatin  recognition property  polygonum cuspidatum Sieb.et Zucc.extracts  solid phase extraction
引用本文:向海艳,范银洲,李曼,卓冬梅,谢扬.选择性富集分离虎杖中白藜芦醇苷的分子印迹聚合物的制备及分子识别性能研究[J].分析测试学报,2013,32(1):45-50.
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