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分子印迹滤过型净化柱净化/分子荧光光谱法测定花生根中白藜芦醇
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作者单位
丘秀珍*,彭翠红,王少玲,郭会时,卢吕泉 韶关学院化学与环境工程学院 
基金项目:广东省自然科学基金(2014A030307024);2015年广东省大学生创新创业立项项目
中文摘要:以白藜芦醇(RES)为模板分子,凹凸棒土(ATP)为载体,β-环糊精(β-CD)和甲基丙烯酸(MAA)为双功能单体,采用表面分子印迹技术制备了白藜芦醇分子印迹聚合物(MIPs)。将制得的分子印迹聚合物作为吸附剂填料,填充滤过型净化柱,用于富集净化花生根中的白藜芦醇,建立了分子印迹滤过型净化柱净化/分子荧光光谱测定花生根中白藜芦醇的新方法。在最优化条件下,分子印迹聚合物对RES具有良好的选择性,最大吸附容量可达12.78 mg/g,白藜芦醇在0.1~100 μg/mL浓度范围内呈良好的线性关系(r=0.999 7),方法检出限为0.048 μg/L,样品加标回收率为86.2%~102.1%,相对标准偏差(RSD)为3.5%~5.8%。该方法高效、快速、选择性好,可用于白藜芦醇的快速检测。
中文关键词:白藜芦醇  分子印迹聚合物  滤过型净化柱  分子荧光光谱法  花生根
 
Determination of Resveratrol in Peanut Root Using Molecularly Imprinted Polymers Multiplug Filtration Clean up Column Coupled with Fluorescence Spectrometry
Abstract:New molecularly imprinted polymers(MIPs) were prepared by using attapulgite(ATP) as the support,β-CD and methacrylic acid(MAA) as functional monomer,resveratrol as template molecule through surface molecularly imprinted polymerization technology.The MIPs were used as sorbent filled into the multiplug filtration clean-up(m-PFC) column for resveratrol enrichment and purification.A new method was developed for the determination of resveratrol by using multiplug filtration clean-up column with molecularly imprinted polymers extraction(MIPs-m-PFCE) combined with fluorescence spectrometry.The results showed that the present method has a high selectivity for RES under the optimized experimental conditions,the adsorption capacity of MIPs was 12.78 mg/g.The calibration curve was linear in the range of 0.1-100 μg/mL with a correlation coefficient(r) of 0.999 7.The limit of detection was 0.048 μg/L.The proposed method was successfully applied in the determination of RES in peanut root,with average recoveries of 86.2%-102.1% and relative standard deviations(RSDs) of 3.5%-5.8% at three spiked levels.The developed method is rapid and selective,and is adaptable to the analysis of trace RES in real samples.
Key Words:resveratrol  molecular imprinted polymers  multiplug filtration clean-up column  molecular fluorescence spectrometry  peanut root
引用本文:丘秀珍*,彭翠红,王少玲,郭会时,卢吕泉.分子印迹滤过型净化柱净化/分子荧光光谱法测定花生根中白藜芦醇[J].分析测试学报,2015,34(12):1403-1407.
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