改性纳米金富集-毛细管电泳电化学发光法测定水产品中4种氟喹诺酮类药物残留 |
点此下载全文 |
|
基金项目:国家自然科学基金项目(21465020,21765018);江西省重点研发计划项目(20161BBF60042) |
|
中文摘要:建立了一种利用改性纳米金粒子富集与毛细管电泳-电化学发光(CE-ECL)法测定水产品中4种氟喹诺酮(环丙沙星、恩诺沙星、氧氟沙星、诺氟沙星)类药物残留的分析方法。实验考察了富集条件与CE分离条件,并基于增强Ru(bpy)2+3电化学发光的原理,优化了ECL检测条件。结果表明,在最优条件下,经改性金纳米粒子富集后的4种分析物在0.05~100 μmol/L浓度范围内,其峰高与浓度呈现良好的线性关系,检出限(S/N=3)可达0.2 μmol/L,4种目标物的富集倍数达104~127倍。该方法用于鳗鱼样品的分析,回收率为94.5%~112%,相对标准偏差(RSD)均不大于6.3%。 |
中文关键词:改性纳米金粒子 富集 氟喹诺酮药物 毛细管电泳-电化学发光(CE-ECL) |
|
Determination of Four Fluoroquinolones Drug Residues in Aquatic Products by Capillary Electrophoresis-Electrochemiluminescence with Modified Gold Nanoparticles Enrichment |
|
|
Abstract:A method was established for the determination of 4 fluoroquinolones,ie.ciprofloxacin(CIP),enrofloxacin(EN),ofloxacin(OF) and norfloxacin(NOR) in aquatic products by capillary electrophoresis-electrochemical luminescence(CE-ECL) with modified gold nanoparticle enrichment.The conditions for enrichment and CE separation were studied,and the conditions for ECL detection were also investigated based on the principle of enhanced Ru(bpy)2+3 electrochemiluminescence.Under the optimal conditions,there were good linearities for the 4 analytes enriched with modified gold nanoparticles in the concentration range of 0.05-10.0 μmol/L.The detection limits(S/N=3) were low to 0.02 μmol/L and the enrichment times were 104-127.This method was used in the analysis of 4 analytes in actual eels samples with recoveries of 94.5%-112% and relative standard deviations(RSDs) not more than 6.3%. |
Key Words:modified gold nanoparticles enrichment fluoroquinolones drug capillary electrophoresis-electrochemical luminescence(CE-ECL) |
引用本文:陈宗保,王星,尹月春,陈国南.改性纳米金富集-毛细管电泳电化学发光法测定水产品中4种氟喹诺酮类药物残留[J].分析测试学报,2019,38(2):176-181. |
摘要点击次数: 2278 |
全文下载次数: 934 |
查看全文 下载PDF阅读器 |
|