| UPLC-DAD法同时测定预调鸡尾酒中24种水溶性合成色素 |
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| 基金项目:国家科技支撑项目(2015BAK45B01)；科技部公益性行业科研专项(2012104001) |
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| 中文摘要:建立了预调鸡尾酒中24种水溶性合成色素的超高效液相色谱-二极管阵列检测器(UPLC-DAD)分析方法，对流动相、色谱柱、洗脱梯度等色谱条件进行了优化，确定最佳分离条件为：Waters BEH C18色谱柱(2.1 mm×50 mm，1.7 μm)，流动相为10 mmol/L乙酸铵(pH 6.25)和甲醇-乙腈(2∶8,体积比)，采用梯度洗脱，在16 min内实现24种色素的快速分离。24种色素在0.01~50.0 mg/L范围内具有良好的线性关系(r2> 0.998 0)，方法检出限为0.66~27.78 μg/L，定量下限为2.19~92.59 μg/L；日内相对标准偏差（RSD）为0.04%~5.3%，日间RSD为0.08%~6.4%；回收率为53.4%~114%。该方法具有所测色素种类多、分析时间短、检出限低等优势，已成功应用于市售预调鸡尾酒样品的检测。 |
| 中文关键词:合成色素 预调鸡尾酒 超高效液相色谱-二极管阵列检测器(UPLC-DAD) |
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| Simultaneous Determination of 24 Water soluble Synthetic Colors in Premade Cocktails by Ultra performance Liquid Chromatography with Diode Array Detector |
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| Abstract:A method for the simultaneous determination of 24 water soluble synthetic colors in premade cocktails was developed using ultra performance liquid chromatography with diode array detector(UPLC-DAD).In this paper,the chromatographic conditions for mobile phase,column and eluting gradient were optimized,and the best separation conditions were as follows:column:a Waters BEH C18 column(2.1 mm×50 mm,1.7 μm),mobile phase:10 mmol/L ammonium acetate solution (pH 625) and methanol-acetonitrile(2∶8,by volume),eluting by a gradient program.Under the optimized conditions,all the water soluble synthetic colors could be rapidly separated within 16 min.There were good linear relationships for the analytes in the concentration range of 0.01-50.0 mg/L with their correlation coefficients(r2) greater than 0.998 0.The limits of detection(LOD,S/N=3) and the limits of quantitation were in the ranges of 0.66-27.78 μg/L and 2.19-92.59 μg/L,respectively.The intra day and inter day RSD for the analytes were in the ranges of 0.04%-5.3% and 0.08%-6.4%,respectively.The recoveries for the synthetic colors ranged from 53.4% to 114%.With the advantages of many kinds of dyes detected,short analysis time and low detection limit,the method was successfully applied in the determination of cocktail samples on the market. |
| Key Words:synthetic colors premade cocktails ultra performance liquid chromatography with diode array detector(UPLC-DAD) |
| 引用本文：武太鹏,张京晨,马康.UPLC-DAD法同时测定预调鸡尾酒中24种水溶性合成色素[J].分析测试学报,2019,38(2):207-212. |
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