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DNA碱基激光拉曼光谱检测方法的研究
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作者单位
李振庆,何志祥,尚华,张大伟,山口佳则 1.上海理工大学光电信息与计算机工程学院教育部医用光学仪器与设备重点实验室2.开封市儿童医院3.大阪大学先进光子研究中心 
基金项目:上海市2018年度“科技创新行动计划”(18441900400)
中文摘要:以激光拉曼光谱仪采集腺嘌呤(A)、鸟嘌呤(G)、胞嘧啶(C)、胸腺嘧啶(T)的拉曼光谱图,分析了各碱基谱峰归属,并依据归属结果检测混合碱基组成。结果显示:4种碱基的特征峰集中在175~1 800 cm-1。相同积分时间和激光功率条件下,该波段内嘌呤碱基的激光拉曼光谱峰高于嘧啶碱基的谱峰。腺嘌呤、鸟嘌呤、胞嘧啶、胸腺嘧啶分别在723、648、791、1 368 cm-1处的谱峰最强,利用这些特征峰能在2 min内鉴别出混合碱基中的各碱基。
中文关键词:激光拉曼光谱  碱基  特征峰
 
Study on Detection of DNA by Laser Raman Spectrometry
Abstract:A method of laser Raman spectrometry was developed for the detection of DNA bases.The Raman spectra of adenine(A),guanine(G),cytosine(C) and thymine(T) were collected with laser Raman spectrometer in this work.The characteristic peaks in the spectra for each DNA base were analyzed,which were used to determine the components of DNA base mixtures.Results demonstrated that the characteristic peaks mainly ranged from 175 cm-1 to 1 800 cm-1.The peaks of A and G were higher than those of C and T when the integration time and laser power were certain.The strongest Raman signals for A,G,C,and T are at 723,648,791 and 1 368 cm-1,respectively.Each DNA base could be determined by using the characteristic peaks from the mixed DNA bases within 2 min.
Key Words:laser Raman spectrometry  DNA base  characteristic peak
引用本文:李振庆,何志祥,尚华,张大伟,山口佳则.DNA碱基激光拉曼光谱检测方法的研究[J].分析测试学报,2019,(2):235-239.
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