卟啉多孔化合物的合成及其在光电免疫传感器中的应用 |
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基金项目:国家自然科学基金资助项目(21465026;21765026) |
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中文摘要:构建了以卟啉多孔化合物-金纳米颗粒(Au NPs@PAF)为固定基质的无标记型C-反应蛋白(CRP)光电免疫传感器。在最优条件下,采用计时电流法实现了对CRP的定量检测。该传感器在CRP质量浓度0.05 ~60 ng/mL范围内与光电流有较好的线性关系,检出限为0.017 ng/mL,相关系数为0.994 6,平均回收率为102%,具有良好的选择性,为C-反应蛋白的检测提供了一种灵敏的方法。 |
中文关键词:卟啉多孔材料 光电免疫传感器 C-反应蛋白 应用 |
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Synthesis of Porous Porphyrin Compound and Its Application in Photoelectric Immunosensor |
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Abstract:In this paper,a label free C-reactive protein(CRP)photoelectricimmunosensor was constructed using porous porphyrin compound-Au nanoparticles(Au NPs@PAF)as an immobilized substrate.Under the optimal conditions,the CRP was quantitatively detected by chronograph current method.There was a good linear relationship for the sensor versus photocurrent in the range of 0.05-60 ng/mL,with a linear correlation coefficient of 0.994 6.The limit of detection was 0.017 ng/mL,and the average recovery was 102%.This sensor provided a sensitive method for the detection of C-reactive protein with good selectivity. |
Key Words:porphyrin porous materials photoelectric immunosensor C-reactive protein application |
引用本文:王敏,孟爽,谷梦巧,迟宽能,马玉婵,邓燕,李德蕾,张茜,胡蓉,杨云慧.卟啉多孔化合物的合成及其在光电免疫传感器中的应用[J].分析测试学报,2019,38(3):283-288. |
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