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辣根过氧化物酶直接电化学传感器检测过氧化物的研究
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作者单位
曹嫱,杨绍明,杨杰,张小荣,柏朝朋,滕渝 华东交通大学材料学院 
基金项目:国家自然科学基金项目(21465012);江西省自然科学基金项目(20171BAB203017)
中文摘要:该文以高比表面积的泡沫镍电极(Ni foam)为基础,通过电沉积碳纳米管(CNTs)制备了CNTs/Ni foam。然后在十六烷基三甲基溴化铵(CTAB)的辅助下,通过一步法电沉积纳米金(AuNPs)将辣根过氧化物酶(HRP)固定到电极表面,制备了HRP-AuNPs/CNTs/Ni foam直接电化学酶传感器。并采用SEM、能谱(EDS)和电化学方法对该电极进行了表征,优化了测试电位和pH值,将该传感器对过氧化氢及2种有机过氧化物进行了检测。结果表明,该传感器性能良好,对过氧化氢、过氧化氢异丙苯、2 过氧化丁酮具有良好的催化检测性能,其检出限分别为1.2×10-7、4.5×10-7、2.5×10-7 mol/L。
中文关键词:过氧化物  辣根酶  碳纳米管  传感器
 
Research on Detection of Peroxides with Horseradish Peroxidase Direct Electrochemical Sensor
Abstract:Based on the surface of nickel foam electrode(Ni foam)with high specific surface area,a carbon nanotubes(CNTs)/Ni foam was prepared through the electrodeposition of CNTs.With the help of cetyltrimethylammonium bromide(CTAB),horseradish peroxidase(HRP)was immobilized onto the surface of CNTs/Ni foam by one-step electrodeposition of Au nanoparticles(AuNPs),and an HRP-AuNPs/CNTs/Ni foam direct electrochemical enzyme sensor was prepared.SEM,electron dispersive spectroscopy(EDS)and electrochemical methods were adopted to characterize the modified electrode,and the test conditions for pH value and work potential were also optimized.The sensor was applied in the detection of hydrogen peroxide and two organic peroxides.Results showed that the sensor has a good catalytic detection performance toward hydrogen peroxide,cumene hydroperoxide and 2-butanone peroxide with the detection limits of 1.2×10-7,4.5×10-7 and 2.5×10-7 mol/L,respectively.
Key Words:peroxides  horseradish peroxidase  carbon nanotubes  sensor
引用本文:曹嫱,杨绍明,杨杰,张小荣,柏朝朋,滕渝.辣根过氧化物酶直接电化学传感器检测过氧化物的研究[J].分析测试学报,2019,(3):345-349.
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