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以硼和苯基共掺杂的石墨相氮化碳纳米片为模拟酶的过氧化氢荧光传感器
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作者单位
钟坚海,陈靖,黄建立,陈宜,张艳燕,温建荣,苏雪香,郭良洽 1.龙岩出入境检验检疫局综合技术服务中心2.福州大学食品安全与生物分析教育部重点实验室3.福建省粮油质量监测所 
基金项目:国家质量监督检验检疫总局科技计划项目(2016IK036);福建省科技厅引导性项目(2015Y0045);国家自然科学基金项目(21577017)
中文摘要:通过热聚合氰胺和3 氨基苯硼酸并结合超声剥离的方法制备了具有辣根过氧化物模拟酶性质的硼和苯基共掺杂的石墨相氮化碳纳米片(BP-C3N4)。与辣根过氧化物酶类似,BP-C3N4可催化过氧化氢氧化荧光底物Amplex Ultrared试剂(AUR),生成强荧光的试卤灵,基于此设计了一种以BP-C3N4为模拟酶的过氧化氢荧光传感器。进一步研究了反应时间、pH值、反应温度对荧光传感体系的影响。在最佳试验条件下,该体系对过氧化氢的线性范围为0~100 μmol/L(r2=0.992 2),检出限为1.1 μmol/L。将该方法用于实际样品中过氧化氢含量的检测,检测结果与现有方法一致。
中文关键词:石墨相氮化碳  共掺杂  过氧化物模拟酶  过氧化氢  荧光传感器
 
A Fluorescence H2O2 Sensor Based on Boron and Phenyl doped Graphitic Carbon Nitride(g-C3N4) Nanosheets as Peroxidase Mimic
Abstract:A novel “turn on” fluorescence sensor for H2O2 detection was developed based on boron and phenyl doped graphitic carbon nitride nanosheets(BP-C3N4) as peroxidase mimic.The BP-C3N4 with peroxidase-like activity were prepared by thermal polymerization of cyanamide with 3-aminobenzeneboronic acid followed by ultrasonic exfoliation.Similar to horseradish peroxidase,the BP-C3N4 could catalyze the oxidation of amplex ultrared reagent(AUR) by H2O2 to generate the resorufin with strong fluorescence.Parameters for the peroxidase like catalytic reaction,including response time,pH value and temperature were further investigated.Under the optimal conditions,the obtained fluorescence signal showed a good linear response to H2O2 concentration over the range of 0-100 μmol/L,with a correlation coefficient(r2) of 0.992 2 and a limit of detection of 1.1 μmol/L.The fluorescence sensor was applied in the detection of H2O2 residue in real samples,and the results were consistent with those of the existed method.
Key Words:graphitic carbon nitride  codope  peroxidase mimic  H2O2  fluorescence sensor
引用本文:钟坚海,陈靖,黄建立,陈宜,张艳燕,温建荣,苏雪香,郭良洽.以硼和苯基共掺杂的石墨相氮化碳纳米片为模拟酶的过氧化氢荧光传感器[J].分析测试学报,2019,(3):350-354.
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