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纸芯片显色法用于L-半胱氨酸的快速测定
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作者单位
张剑,谢婷婷,杨玉莹,刘春叶,Frank AGOMEZ,Wilson LEE,肖倩 1.西安医学院药学院2.加州州立大学化学与生物化学系 
基金项目:国家自然科学基金项目(81202492);陕西省教育厅项目(18JK0670);西安医学院国家基金培育项目(2017GJFY06);西安医学院振兴人才项目(2018XNRC03);西安医学院博士科研启动基金(2018DOC17)
中文摘要:该文以5,5'-二硫硝基苯甲酸(DTNB)为含巯基化合物的衍生化试剂,建立了其纸芯片快速检测新方法。显色剂DTNB和样品在显色区相遇并显色,用手机拍照记录,采用Photoshop软件分析显色强度,进行比色检测,并以L-半胱氨酸(L-Cys)为例,优化得到纸芯片检测L-Cys的最佳显色条件。结果显示,L-Cys浓度在0.01~0.1 mmol/L范围内与显色强度呈良好的线性关系(r=0.999 8),检出限达0.001 mmol/L。多种氨基酸和干扰离子对L-Cys在纸芯片上的测定干扰不超过±5%。采用该方法测得牛血清样品中L-Cys含量为0.013 mmol/L,加标回收率为99.1%~ 103%,该方法检测L-Cys具有操作简单、耗样量少、检测快速、重现性好等优点。
中文关键词:纸芯片  巯基化合物  5,5'-二硫硝基苯甲酸(DTNB)  比色法
 
Rapid Detection of L-Cysteine by Colorimetric Method Based on Paper-based Microfluidic Chip
Abstract:A new colorimetric method based on paper chip was established for the rapid detection of L-Cysteine(L-Cys) using 5,5'-dithiobis-(2-nitrobenzoic acid)(DTNB) as derivatizing agent.A normal phone was used to record the color intensity.The color intensity was analyzed by Photoshop software and used to calculate the concentration of thiol contained molecules in samples.The optimum coloration conditions for the detection were investigated.Results showed that there was a good linear relationship between color intensity and L-Cys concentration in range of 0.01-0.1 mmol/L with the linear correlation coefficient of 0.999 8 and the detection limit of 0.001 mmol/L.In the range of ±5%,the interference of several amino acids and ions on the determination of L-Cyse was small.The method was applied in the detection of L-Cys content in bovine serum sample with a result of 0.013 mmol/L and a spiked recovery of 99.1%-103%.Results showed that this method has high selectivity and reproducibility.The colorimetric method based on paper based microfluidic chip has several advantages such as simplicity,low reagent consumption,rapidness and repeatability.
Key Words:paper based microfluidic chip  thiol contained molecules  5,5'-dithiobis-(2-nitrobenzoic acid)(DTNB)  colorimetric method
引用本文:张剑,谢婷婷,杨玉莹,刘春叶,Frank AGOMEZ,Wilson LEE,肖倩.纸芯片显色法用于L-半胱氨酸的快速测定[J].分析测试学报,2019,(4):477-481.
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