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子体铅诱导T30695构象改变N-甲基卟啉二丙酸Ⅸ(NMM)荧光探针检测氡
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作者单位
刘红文,刘士蒙,李诗雅,杨桂英,刘然,吕昌银 南华大学公共卫生学院 
基金项目:国家自然科学基金资助项目(81473021)
中文摘要:氡衰变形成稳定的子体铅只需约3.82 d,且衰变产生的210Pb非常稳定,其半衰期为21年。基于氡辐射衰变最终形成稳定的子体铅的特点,探讨了子体铅诱导富鸟嘌呤核苷酸序列形成G-四链体的特性,建立了一种基于子体铅诱导T30695构象改变的荧光传感器。氡的子体铅诱导T30695形成的G-四链体结构能与N-甲基卟啉二丙酸Ⅸ(NMM)荧光染料结合发出较强荧光,在一定范围内,荧光强度与Pb2+的浓度和氡累积辐射剂量浓度成正相关。Pb2+离子浓度在5.13×10-9~1.25×10-7mol/L范围内与体系的ΔIF值呈现良好的线性关系,回归方程为ΔIF=77.30+5.05c,r=0.995 0,对铅的检出限为1.54×10-9mol/L,对氡的检出限为1.07×104 Bq?h/m3。
中文关键词:  子体铅  核酸适配体  G-四链体  构象转变  荧光传感
 
Detection of Radon Using a Label-free Fluorescence Sensor Based on Combination of Pb2+-induced Allosteric T30695 with N-Methyl Mesoporphyrin Ⅸ
Abstract:A fluorescent sensor was fabricated for the detection of radon based on the daughter lead inducing G-rich aptamer T30695 to form G-quadruplex.According to the characteristics of radon radioactive decay,it only takes about 3.82 days for radon to decay and form a stable daughter lead,and 210Pb derived from radon decay is very stable.The half-life period of the stable daughter(210Pb)resulted from radon is 21 years,and the lead daughter product could be used as the target for detection.When there was no lead in the system,T30695 combined with its complementary chain to form a double-stranded DNA structure,and the fluorescent signal of N-methyl mesoporphyrin IX(NMM)was weak.When the system contained daughter lead,aptamer T30695 was induced to form a G-quadruplex structure,and NMM combined with G-quadruplex structure to produce strong fluorescence.Within a certain range, the fluorescence intensity is positively correlated with the concentration of Pb2+ and radon.There existed a linear relationship btween the fluorescence value(ΔIF)of reaction system and Pb2+ concentration in the range of 5.13×10-9-1.25×10-7 mol/L,with a linear regression equation of ΔIF=77.30+5.05c,a detection limit of 1.54×10-9 mol/L and a correlation coefficient(r)of 0.995 0.The detection limit for radon was 1.07×104 Bq?h/m3.
Key Words:radon  lead  aptamer  G-quadruplex  conformation transformation  fluorescence sensor
引用本文:刘红文,刘士蒙,李诗雅,杨桂英,刘然,吕昌银.子体铅诱导T30695构象改变N-甲基卟啉二丙酸Ⅸ(NMM)荧光探针检测氡[J].分析测试学报,2019,(6):643-648.
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