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顶空-固相微萃取法与水蒸气蒸馏法提取沙棘挥发油组分的比较研究
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张鹏云,李蓉,卢俊文,张朋杰,陈丽斯,张峰 1.中山出入境检验检疫局检验检疫技术中心2.广东药科大学公共卫生学院3.中国检验检疫科学研究院食品安全研究所 
基金项目:国家重点研发计划项目(2017YFF0211000)
中文摘要:优化了顶空-固相微萃取法(HS-SPME)提取沙棘挥发性成分的条件,并采用气相色谱-质谱联用技术(GC-MS)分别对HS-SPME法和水蒸气蒸馏法(SD)的提取物进行分析。结果显示,在萃取温度为70 ℃,萃取时间为50 min,解吸时间为7 min,平衡时间为20 min条件下,HS-SPME法鉴定出76种组分,占挥发性物质总量的90.19%,主要成分为酯类、醛类和酮类;而SD法提取物共鉴定出56种组分,占挥发性物质总量的91.98%,主要成分为酯类。2种方法共有组分为20种。两种方法提取的沙棘挥发油组分的种类及含量差异较大,HS-SPME法更适合沙棘挥发性组分的快速检测。
中文关键词:沙棘  顶空固相微萃取法  水蒸气蒸馏法  挥发性成分  气相色谱-质谱(GC-MS)
 
Comparison of Volatile Components in Hippophae Rhamnoides L.Extracted by Headspace Solid Phase Microextraction and Steam Distillation
Abstract:The conditions for extracting volatile components in Hippophae rhamnoides L.by headspace solidphase microextraction(HS-SPME) were optimized.Gas chromatography-mass spectrometry(GC-MS) was used to detect the extracts by HS-SPME and steam distillation(SD).Results showed that the optimum conditions for HS-SPME were as follows:the extraction temperature and the extraction time were 70 ℃ and 50 min,respectively,and the desorption time and the equilibrium time were 7 min and 20 min,respectively.Under this condition,76 components were identified from the HS-SPME extracts,accounting for 90.19% of the total,and the main components were esters,aldehydes and ketones,respectively,while 56 components were identified from the SD extracts,accounting for 91.98% of the total,and the main components were esters.20 common components were detected in both the extracts.There are great differences in the types and contents of volatile oil in Hippophae rhamnoides L.extracted by the two extraction methods.The HS-SPME is more suitable for the rapid detection of volatile components in Hippophae rhamnoides L.
Key Words:Hippophae rhamnoides L.  headspace solidphase microextraction(HS-SPME)  steam distillation(SD)  volatile components  gas chromatography-mass spectrometry(GC-MS)
引用本文:张鹏云,李蓉,卢俊文,张朋杰,陈丽斯,张峰.顶空-固相微萃取法与水蒸气蒸馏法提取沙棘挥发油组分的比较研究[J].分析测试学报,2019,(6):699-705.
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