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基于UPLC-LTQ-Orbitrap-MS技术快速解析杜仲颗粒化学成分
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作者单位
马永青,王璐,刘颖,李欣欣,刘永利 1.河北省药品检验研究院2.河北省食品药品审评认证中心3.中国药科大学中药学院 
基金项目:河北省科技项目(16272511)
中文摘要:建立了定性分析杜仲颗粒化学成分的超高效液相色谱-线性离子阱-静电场轨道阱组合式高分辨质谱(UPLC-LTQ-Orbitrap-MS)方法。杜仲颗粒采用甲醇超声提取,Thermo Scientific Syncronis C18色谱柱(100 mm×21 mm,19 μm)分离,以乙腈-01%甲酸溶液为流动相进行梯度洗脱。利用LTQ-Orbitrap-MS负离子模式采集,通过对照品比对及高分辨质谱数据解析,鉴定了25种化学成分,包括8种环烯醚萜类、8种黄酮类、5种苯丙素类、3种木脂素类化合物及1种非还原性双糖。该方法简便、快速、准确,可为进一步分析杜仲颗粒的药效物质基础提供依据。
中文关键词:杜仲颗粒  超高效液相色谱-线性离子阱-静电场轨道阱组合式高分辨质谱(UPLC-LTQ-Orbitrap-MS)  定性分析  黄酮类  环烯醚萜类
 
Rapid Identification of Chemical Compositions in Duzhong Granules by UPLC-LTQ-Orbitrap-MS Technique
Abstract:An ultra high performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometric(UPLC-LTQ-Orbitrap-MS) method was developed for the qualitative analysis of main chemical constituents in Duzhong granules,a Chinese medicine.The sample was ultrasonically extracted with methanol,then separated on a Thermo Scientific Syncronis C18 chromatographic column(100 mm×21 mm,19 μm) by gradient elution with acetonitrile-01% formic acid as mobile phase.The data were collected by using LTQ-Orbitrap-MS in negative ion mode.According to the contrast of the reference standards and the accurate masses of molecules,a total of 25 compounds in Duzhong granules were identified,including 8 iridoids,8 flavonoids,5 phenylpropanoids,3 lignans and 1 non reducing disaccharide.Results showed that,with the advantages of simplicity,rapidness and accuracy,the developed method was suitable for the identification of chemical constituents in Duzhong granules,which provided a reference for the pharmacological basis of further analysis of Duzhong granules.
Key Words:Duzhong granules  ultra high performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry(UPLC-LTQ-Orbitrap-MS)  qualitative analysis  flavonoids  iridoids
引用本文:马永青,王璐,刘颖,李欣欣,刘永利.基于UPLC-LTQ-Orbitrap-MS技术快速解析杜仲颗粒化学成分[J].分析测试学报,2019,(7):817-822.
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