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基于G-四链体-氯化血红素DNA酶比色法测定银离子和汞离子传感器的构筑
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作者单位
肖志友,司恒丹,邓兰清,龙丽,居荣梅,张鑫,刘益飞 贵州理工学院化学工程学院 
基金项目:国家自然科学基金资助项目(21768002);贵州省科学技术基金资助项目(黔科合J字[2013]2263号);贵州理工学院高层次人才科研启动经费项目(XJGC20181213);贵州理工学院大学生创新训练项目(201814440229)
中文摘要:利用G 四链体DNA(5′ CTGGGAGGGAGGGAGGGA 3′)与氯化血红素结合形成G 四链体-Hemin DNA酶,其能高效催化H2O2氧化反应底物由无色变为绿色,当溶液中有Ag+或Hg2+存在时会阻碍该DNA酶的形成,导致绿色溶液变浅。基于此,建立了比色法测定Ag+和Hg2+的传感器。在最佳实验条件下,溶液的吸光度与Ag+和Hg2+浓度分别在1000~1 0000 nmol/L和800~8000 nmol/L范围内具有良好的线性关系,检出限(3δ/Slope)分别为559 nmol/L和 643 nmol/L。该方法具有较好的选择性,采用该方法对实际样品进行测试,结果满意。
中文关键词:G 四链体-氯化血红素DNA酶  比色法测定  银离子  汞离子  传感器
 
Fabrication of a Sensor for Colorimetric Determination of Silver and Mercury Ions Based on G quadruplex-Hemin DNAzymes
Abstract:A sensor for colorimetric determination of silver and mercury ions based on G quadruplex-hemin DNAzymes was fabricated.The G quadruplex-hemin DNAzymes was formed by the combination of G quadruplex(5′ CTGGGAGGGAGGGAGGGA 3′) with hemin,which could catalyze H2O2 to oxide the substrate efficiently,and make the colorless solution become green.The presence of Ag+ or Hg2+ in the solution would hinder the formation of the DNAzyme,and the solution color became lighter.Under the optimal experimental conditions,there were good linear relationships for the absorbance of the solution with Ag+ and Hg2+ in the concentration ranges of 1000-1 0000 nmol/L and 800- 8000 nmol/L,with detection limits(3δ/Slope) of 559 nmol/L and 643 nmol/L,respectively.This method was of good selectivity,and was applied in the detection of real samples with satisfactory results.
Key Words:G quadruplex-hemin DNAzymes  colorimetric determination  silver ion  mercury ion  sensor
引用本文:肖志友,司恒丹,邓兰清,龙丽,居荣梅,张鑫,刘益飞.基于G-四链体-氯化血红素DNA酶比色法测定银离子和汞离子传感器的构筑[J].分析测试学报,2019,38(7):840-844.
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