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生姜精油的超高效合相色谱特征图谱研究
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作者单位
宋伟,韩芳,丁磊,周典兵,贾学颖,吕亚宁,邓晓军,郑平,盛旋,胡艳云 1.安徽出入境检验检疫局检验检疫技术中心2.食品安全分析与检测安徽省重点实验室3.上海检验检疫科学技术研究院 
基金项目:安徽省对外科技合作项目(1804b06020349);中央引导地方科技发展专项(YDZX20173100004528);原国家质检总局科研计划项目(2016IK001)
中文摘要:应用超高效合相色谱(UPC2)技术研究了生姜精油的特征图谱。生姜精油经正庚烷稀释,以超临界CO2为流动相,乙腈为助溶剂,采用超高效合相色谱梯度洗脱分离,流速为08 mL/min,二极管阵列(PDA)检测器测定,建立了特征峰PDA光谱谱图库及安徽产生姜精油的标准对照特征图谱。通过特征峰PDA 光谱图检索识别及色谱图相似度评价,10份安徽产生姜精油的样品谱图与标准对照特征图谱的相似度均大于09。各共有指纹峰相对保留时间和相对峰面积的相对标准偏差(RSD)均小于30%,方法精密度、重现性、稳定性良好,符合特征图谱评价要求。通过超高效合相色谱特征图谱采集,结合聚类分析、主成分分析,对20批不同来源的生姜精油样品成功进行了区分。该研究为生姜精油的质量控制及产地溯源提供了一种简便、快速的新方法。
中文关键词:生姜精油  超高效合相色谱(UPC2)  特征图谱
 
Study on Specific Chromatogram of Ginger Essential Oil by Ultra Performance Convergence Chromatography
Abstract:Specific chromatogram of ginger essential oil was studied by ultra performance convergence chromatographic(UPC2) method.The ginger essential oil was diluted with n heptane,and separated on UPC2 system with photo diode array(PDA) detector,using supercritical CO2 as mobile phase and acetonitrile as cosolvent at a flow rate of 08 mL/min by gradient elution.A spectral library for characteristic peaks and standard specific chromatogram of ginger essential oil from Anhui was established.By comparison with the characteristic peaks from PDA library and similarity evaluation,the resemblance of specific chromatogram was found higher than 09 among the ginger essential oil from Anhui and the standard control.The relative standard deviations(RSD) for relative retention time and relative peak area of common peaks were less than 30%.This method has good precision,reproducibility and stability,and could meet the requirements for the evaluation of specific chromatogram.Coupled with cluster analysis and principal component analysis,the UPC2 specific chromatogram method was successfully used to distinguish 20 samples from different habitats.This study provided a novel,convenient and rapid method for the quality control and origin traceability of ginger essential oil.
Key Words:ginger essential oil  ultra performance convergence chromatography(UPC2)  specific chromatogram
引用本文:宋伟,韩芳,丁磊,周典兵,贾学颖,吕亚宁,邓晓军,郑平,盛旋,胡艳云.生姜精油的超高效合相色谱特征图谱研究[J].分析测试学报,2019,(7):845-848.
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