| 分离式阴极光电化学检测致病菌的新方法研究 |
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| 基金项目:国家自然科学基金项目（21275065，21575052） |
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| 中文摘要:利用液相法合成了水溶性的巯基乙酸修饰的硒化铅（PbSe）量子点（QDs），并将其修饰至氧化铟锡（ITO）电极，制备了ITO/PbSe电极。Zn2+可与电极上修饰的PbSe QDs进行离子交换形成ZnSe/PbSe/ZnSe量子阱（QW），促进电子-空穴分离，提高ITO/PbSe电极的阴极光电流。以抗菌肽为识别探针，开发出一种分离式光电化学（PEC）检测大肠杆菌O157∶H7（E.coli O157∶H7）的方法。该方法避免了生物分子固定在电极上所导致的PEC信号传输的延误，具有更好的检测效果。E.coli O157∶H7的检测范围为10.0~5.0×106 CFU/mL，检出限为4.0 CFU/mL，回收率为94.7%~104%，相对标准偏差为1.9%~2.8%。 |
| 中文关键词:光电化学分析 ZnSe/PbSe/ZnSe量子阱 大肠杆菌O157∶H7 |
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| A Novel Split-type Cathodic Photoelectrochemical Method for Detection of Pathogenic Bacteria |
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| Abstract:A split-type photoelectrochemical（PEC）method was developed for the detection of Escherichia coli O157∶H7（E.coli O157∶H7）with antibacterial peptide as a recognizer.Firstly,water soluble thioglycolic acid capped lead selenide quantum dots（PbSe QDs）were synthesized by a simple water bath method,then PbSe QDs were assembled on surface of indium tin oxide（ITO）electrode through electrostatic interaction,and the Pb2+ on surface of PbSe QDs could be exchanged with Zn2+ to form a ZnSe/PbSe/ZnSe quantum well（QW）,which could promote the electron-hole separation and improve the cathode photocurrent on the ITO/PbSe electrode.This method avoided the immobilized biomolecules on the electrodes to retard the PEC signal,and showed a highly efficient detection.The detection range of the method for E.coli O157∶H7 ranged from 10.0 CFU/mL to 5.0×106 CFU/mL with a detection limit of 4.0 CFU/mL.The recoveries varied from 94.7% to 104% with relative standard deviations of 1.9%-2.8%. |
| Key Words:photoelectrochemical sensor ZnSe/PbSe/ZnSe quantum well Escherichia coli O157∶H7 |
| 引用本文：杨高霞，董玉明，王光丽.分离式阴极光电化学检测致病菌的新方法研究[J].分析测试学报,2019,38(8):897-904. |
| 摘要点击次数: 2020 |
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