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微波一步法制备氮掺杂碳点及其用于多巴胺的检测研究
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作者单位
孟铁宏,李春荣,王恒,姜艳萍,赵鸿宾,余跃生,胡先运 1.黔南民族医学高等专科学校
2.贵州省中国科学院天然产物化学重点实验室 
基金项目:国家自然科学基金项目(81860317,21545006);贵州省自然科学基金(黔科合支撑[2019]2821号,黔科合基础[2019]1301号,黔科合基础[2016]1136号);贵州省中医药管理局中医药、民族医药科学技术研究项目(QZYY-2018-99);黔南州科技计划项目(黔南州科合社字[2016]1号,黔南科合工字[2016]12号);黔南民族医学高等专科学校基金(QNYZ201605,QNYZ201801,QNYZ201807
中文摘要:以柠檬酸为碳源,尿素为氮源,采用微波一步法制备得到稳定性高,水溶性好的蓝色荧光的氮掺杂碳点。并将该氮掺杂碳点经透射电子显微镜(TEM)、原子力显微镜(AFM)、X-射线光电子能谱(XPS)和红外光谱(IR)表征,发现其粒径为0.5~5 nm,平均粒径2.6 nm,富含羟基、羧基和氨基等官能团。在优化实验条件下,多巴胺浓度在2.5~37.5 μmol/L范围内与N-CDs的荧光猝灭效率(IF/IF0)呈良好的线性关系,方法的检出限为0.08 μmol/L。采用该方法对正常人尿液中的多巴胺进行测定,其加标回收率为99.5%~106%,RSD为1.8%~2.3%,方法准确度高,结果满意。
中文关键词:微波 一步法  氮掺杂碳点  多巴胺  检测
 
Preparation of Nitrogen Doped Carbon Quantum Dots by Microwave One-step Method and Their Application in Dopamine Detection
Abstract:A kind of nitrogen doped carbon quantum dots(N-CDs) with high stability,good water solubility were prepared by one-step microwave method with citric acid as carbon source and urea as nitrogen source.The prepared N-CDs were characterized by transmission electron microscopy(TEM),atomic force microscopy(AFM),X-ray photoelectron spectroscopy(XPS) and infrared spectroscopy(IR).Results showed that the N-CDs contained a particle size ranging from 0.5 nm to 5 nm with an average particle size of 2.6 nm,which were rich in hydroxyl,carboxyl and amino groups and other functional groups.The N-CDs were used in the detection of dopamine.Under the selected experimental conditions,there was a good linear relationship for the fluorescence quenching efficiency(IF/IF0) of N-CDs with dopamine within the concentration range of 25-375 μmol/L,and the method detection limit was 0.08 μmol/L.This method was applied in the detection of dopamine in urine of normal people with high accuracy and satisfactory results.The average recoveries ranged from 995% to 106% with relative standard deviations(RSDs) of 1.8%-2.3%.
Key Words:microwave one step method  nitrogen doped carbon quantum dots  dopamine  detection
引用本文:孟铁宏,李春荣,王恒,姜艳萍,赵鸿宾,余跃生,胡先运.微波一步法制备氮掺杂碳点及其用于多巴胺的检测研究[J].分析测试学报,2019,(8):953-960.
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