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超临界二氧化碳萃取结合高效液相色谱-串联质谱法测定毛发中11种常见精神药物
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作者单位
徐代化,欧军,陈深树,徐期林,陈垚翰,程良红 1.广东南天司法鉴定所 2.深圳黑尔格科技有限公司 
基金项目:广东省深圳市科技创新委员会2017年技术创新计划技术攻关项目(JSGG20170413100657397)
中文摘要:建立了一种超临界二氧化碳萃取(SFE)结合高效液相色谱-串联质谱(HPLC-MS/MS)测定毛发中11种常见精神药物的方法,并应用于实际毛发样品的检测。剪碎的毛发样品在优化的SFE条件下萃取,提取液经氮吹浓缩,以甲醇定容至100 μL后,进行HPLC-MS/MS测定。结果表明,11种常见精神药物在2~200 ng/mL范围内线性良好,相关系数(r2)大于0.999,方法检出限为0.01~0.05 ng/mg,定量下限为 0.03~0.10 ng/mg;在0.05、0.20、0.40 ng/mg加标水平下,方法的回收率为81.6%~94.8%,相对标准偏差(RSD)为0.50%~3.4%。该方法快速、灵敏、准确,可用于毛发中11种常见精神药物的同时检测。
中文关键词:超临界二氧化碳萃取(SFE)  毛发  精神药物  高效液相色谱-串联质谱(HPLC-MS/MS)
 
Determination of 11 Common Psychotropic Drugs in Human Hair by High Performance Liquid Chromatography-Tandem Mass Spectrometry with Supercritical Fluid Carbon Dioxide Extraction
Abstract:A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) with supercritical fluid carbon dioxide extraction(SFE) was established for the determination of 11 common psychotropic drugs in human hair.The sheared hair samples were extracted by SFE under optimized conditions.The concentrated extract was reconstituted to 100 μL with methanol,then analyzed by HPLC-MS/MS.Results showed that there existed good linear relationships for 11 common psychotropic drugs in the range of 2-200 ng/mL,with correlation coefficients(r2) greater than 0.999.The detection limits and the quantitation limits were in the ranges of 0.01-0.05 ng/mg and 0.03-0.10 ng/mg,respectively.Recoveries at the spiked levels of 0.05,0.20 and 0.40 ng/mg ranged from 816% to 94.8% with relative standard deviations(RSD) of 0.50%-3.4%.The method is rapid,sensitive and accurate,and could be used in simultaneous detection of 11 common psychotropic drugs in human hair.
Key Words:supercritical fluid carbon dioxide extraction(SFE)  human hair  psychotropic drugs  high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)
引用本文:徐代化,欧军,陈深树,徐期林,陈垚翰,程良红.超临界二氧化碳萃取结合高效液相色谱-串联质谱法测定毛发中11种常见精神药物[J].分析测试学报,2019,(8):979-984.
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