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聚合物涂层在毛细管电泳分离蛋白中的研究进展
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作者单位
陈丽娟,闫叶寒,朱海锟,Muhammad Atif 1.皖西学院材料与化工学院2.中国科学技术大学化学与材料科学学院 
基金项目:安徽省自然科学基金(1808085QB44);安徽省教育厅科研基金(KJ2018A0416);皖西学院高层次人才科研启动资金项目(WGKQ201702002)
中文摘要:毛细管电泳具有分析时间短,分离效率高,样品消耗量少等优点,在生物样品分离,特别是蛋白质分析领域有重要应用。然而,毛细管内壁硅羟基的解离给分离结果带来诸多不良影响。聚合物涂层能够抑制蛋白质在毛细管内壁的吸附以及调控电渗流,故对毛细管内壁进行有效修饰能够提高其对蛋白质的分离效率及分离稳定性。该文主要综述了动态及静态聚合物涂层毛细管的最新研究进展,并概述了近些年基于多巴胺/聚多巴胺发展起来的涂层毛细管的研究进展,最后展望了聚合物涂层毛细管的发展趋势。
中文关键词:毛细管电泳  聚合物涂层  蛋白质分离  电渗流
 
Advances in Application of Polymer Coatings in Protein Separation by Capillary Electrophoresis
Abstract:Capillary electrophoresis(CE),a highly mature and versatile technique,has been continually evolving in the last three decades.Capillary electrophoresis has important applications in the field of biological analysis such as protein separation due to its advantages of short separation time,high separation efficiency and low sample consumption,etc.However,many drawbacks have been caused by dissociation of silicon hydroxyl on inner wall of capillary in CE separation process.To overcome these drawbacks associated with CE,modification of capillary inner surfaces by antifouling polymer coatings are chosen.The antifouling polymer modified capillary method could not only regulate the electroosmotic flow,but also benefit the proteins separation.This method could also effectively improve the separation efficiency and separation stability for proteins in CE process.The paper presents a review on the latest research progress of capillary coatings,like dynamic and static polymer coating for protein separation.It also summarizes the research progress of polymer coating capillary based on dopamine/polydopamine anchored.And finally,the prospects of the future development trend of polymer coatings of capillary are discussed.
Key Words:capillary electrophoresis  polymer coatings  protein separation  electroosmotic flow
引用本文:陈丽娟,闫叶寒,朱海锟,Muhammad Atif.聚合物涂层在毛细管电泳分离蛋白中的研究进展[J].分析测试学报,2019,(8):1020-1028.
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