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基于PAC修饰的氧化石墨烯负载纳米Pt构建新型三维过氧化氢电化学传感器
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作者单位
王应霞,于颖,李军,彭全材,俞立东,宋金明 1.中科院海洋研究所所级公共技术服务中心2.青岛大学材料科学与工程学院 
基金项目:山东省自然科学基金资助项目(ZR2016EMB2)
中文摘要:为构建一种新的无酶电化学生物传感器并将其用于细胞中释放的H2O2的有效检测,将聚二烯丙基二甲基氯化铵(PAC)修饰的氧化石墨烯(GO)和纳米Pt通过物理吸附和电化学沉积H2PtCl6共同负载于玻碳电极上,成功制备了Pt/PAC-GO/GCE生物传感器。扫描电镜表明纳米Pt能很好地负载于PAC-GO表面。Pt/PAC-GO/GCE生物传感器对H2O2具有较宽的线性范围(0.04~3.6 μmol/L及4.4~28.6 μmol/L)及较高的电流响应(检出限为0.01 μmol/L)。此外,该生物传感器具有较强的抗干扰能力,已成功用于活细胞H2O2的检测,有望用于医学诊断。
中文关键词:生物传感器  过氧化氢  钠米铂  氧化石墨烯(GO)
 
Construction of a Novel 3D Hydrogen Peroxide Electrochemical Biosensor Based on PAC Modified GO/GCE Supported with Nano-Pt
Abstract:In order to construct a new non enzymatic electrochemical biosensor and use it for the effective detection of the release of H2O2 from cells,the graphene oxide(GO) modified by polydiallyl dimethyl ammonium chloride(PAC) and nano Pt were immobilized on glass carbon electrode(GCE) through the physical adsorption and electrodeposition of H2PtCl6 solution,respectively,and a Pt/PAC-GO/GCE electrochemical biosensor was successfully prepared.Through the characterization of scanning electron microscopy(SEM) and energy dispersive X-ray spectroscopy(EDS),it was observed that the electrodeposited nano Pt were densely covered and well distributed on the PAC-GO surface.Under the optimal experimental conditions,the Pt/PAC-GO/GCE biosensor exhibited a high peak current toward H2O2 and wide linear ranges for H2O2 in the range of 0.04-3.6 μmol/L and 4.4-28.6 μmol/L,with a detection limit of 0.01 μmol/L.Moreover,the Pt/PAC-GO/GCE biosensor exhibited excellent anti-interference capability and reproducibility for the detection of H2O2,and was also successfully used in the detection of H2O2 in different living cell.All these results proved that the prepared biosensor has a potential application in clinical diagnostics.
Key Words:biosensor  H2O2  nano Pt  graphene oxide(GO)
引用本文:王应霞,于颖,李军,彭全材,俞立东,宋金明.基于PAC修饰的氧化石墨烯负载纳米Pt构建新型三维过氧化氢电化学传感器[J].分析测试学报,2019,(9):1029-1035.
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