•  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
期刊检索


检索
检索项:
检索词:
总目录
  • WCSB9︱2019(第九届)世界采样和混样大会 查看全文>>
  • 关于召开第十五届全国青年分析测试学术报告会的通知 查看全文>>
友情链接
通过式固相萃取/超高效液相色谱-串联质谱法快速测定动物源性食品中安乃近代谢物残留量
    点此下载全文
作者单位
张婧雯,张海超,范斌,艾连峰,王敬,李玮,康维钧 1.石家庄海关技术中心2.河北省食品检验研究院3.河北医科大学公共卫生学院 
基金项目:国家自然科学基金项目(81872628)
中文摘要:建立了通过式固相萃取/超高效液相色谱-串联质谱( UPLC-MS /MS) 快速测定动物源性食品中4-甲氨基安替比林(MAA)、4-氨基安替比林(AA)、4-甲酰氨基安替比林(FAA)和4-乙酰氨基安替比林(AAA) 4种安乃近代谢物的方法。样品采用乙腈提取,PRiME HLB固相萃取柱净化,在ACQUITY BEH C18色谱柱上以乙腈和0.1%甲酸水溶液为流动相梯度洗脱,超液相色谱-串联质谱MRM方式进行定量分析。结果表明,4种化合物在0.5~50 μg/L范围内线性关系良好,相关系数(r)大于0.996,方法的检出限(LOD)为2 μg/kg,定量下限(LOQ)为5 μg/kg。 4种化合物在3个加标水平(5、10和50 μg/kg)下的回收率为75.1%~115%,相对标准偏差(RSD)为1.6%~7.4%。该方法简单、快速、准确,可实现动物源性食品中安乃近代谢物的快速测定。
中文关键词:安乃近代谢物  通过式固相萃取  PRiME HLB  超高效液相色谱-串联质谱  动物源性食品
 
Rapid Determination of Dipyrone Metabolite Residues in Foods of Animal Origin by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with a Through-type Solid phase Extraction
Abstract:A method was developed for the rapid detection of 4-methylaminoantipyrine(MAA),4-aminoantipyrine(AA),4-formylaminoantipyrine(FAA) and 4-acetylaminoantipyrine(AAA) in foods of animal origin by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with a through type solid phase extraction.Samples were extracted with acetonitrile,then cleaned up with a PRiME HLB column,finally quantified by UPLC-MS/MS in MRM mode after gradient elution on an ACQUITY BEH C18 column with acetonitrile and 0.1% formic acid as mobile phases.Results showed that the calibration curves for four analytes were linear in the range of 0.5-50 μg/L with correlation coefficients(r) larger than 0.996.The limits of detection(LODs) and the limits of quantitation(LOQs) were 2 μg/kg and 5 μg/kg,respectively.The average recoveries at three spiked levels of 5,10 and 50 μg/kg ranged from 75.1% to 115%,with relative standard deviations(RSD) of 1.6%-7.4%.The proposed method is simple,rapid and accurate,and could be applied in the simultaneous determination of dipyrone metabolite residues in foods of animal origin.
Key Words:dipyrone metabolite  through type solid phase extraction  PRiME HLB  ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS /MS)  foods of animal origin
引用本文:张婧雯,张海超,范斌,艾连峰,王敬,李玮,康维钧.通过式固相萃取/超高效液相色谱-串联质谱法快速测定动物源性食品中安乃近代谢物残留量[J].分析测试学报,2019,(9):1114-1119.
摘要点击次数: 532
全文下载次数: 1546
查看全文  查看/发表评论  下载PDF阅读器