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超高效液相色谱法同时测定洋常春藤中8种成分含量
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作者单位
倪力军,文丹瑶,胡甜甜,高丽丽,胡江宁,金汉台,金志文,张立国 1.华东理工大学化学与分子工程学院2.中国医学科学院药物研究所3.浙江康恩贝中药有限公司 
基金项目:国家科技重大专项重大新药创制项目(2018ZX09721003)
中文摘要:建立了同时测定洋常春藤中绿原酸、隐绿原酸、芦丁、烟花苷、常春藤皂苷C、常春藤皂苷D、常春藤皂苷B和α 常春藤皂苷8种成分的超高效液相色谱法(UHPLC)。以80%甲醇为溶剂,将药材粉末于85 ℃、料液比1∶100条件下水浴回流1 h,制备供试品溶液;采用Agilent ZORBAX Eclipse Plus-C18(2.1 mm×100 mm,1.8 μm)色谱柱,以乙腈-0.05%磷酸溶液为流动相进行梯度洗脱。结果表明,上述8种成分均获得良好的分离度;仪器精密度、方法重复性的相对标准偏差(RSD)均小于3.0%;样品溶液在室温条件下24 h内稳定;8种成分在对应质量浓度范围内线性关系良好(r≥0.999 5),检出限为0.40~10.58 μg·mL-1,定量下限为1.31~34.61 μg·mL-1,平均回收率为97.3%~108%,RSD(n=6)为0.51%~3.2%。该方法适用于洋常春藤中上述8种化学成分的定量分析。
中文关键词:洋常春藤  超高效液相色谱(UHPLC)  常春藤皂苷  有机酸  黄酮类化合物
 
Simultaneous Detection of 8 Components in Hedera Helix L by Ultra High Performance Liquid Chromatography
Abstract:A method of ultra high performance liquid chromatography(UHPLC) was developed for the simultaneous determination of eight components,ie.chlorogenic acid,cryptochlorogenic acid,rutin,nicotifiorin,hederacoside C,hederacoside D,hederasaponin B and α hederin in Hedera helix L..The dry leaves passed through 40 mesh sieve were put in 80% methanol with solid-liquid ratio of 1∶100.The solution then was refluxed by water bath for 1 h at 85 ℃ to prepare the test solution.The chromatographic separation was performed on an Agilent ZORBAX Eclipse Plus-C18(2.1 mm×100 mm,1.8 μm) column by gradient elution with acetonitrile and 0.05%phosphoric acid as mobile phases.Results indicated that the eight components in Hedera helix L.were well separated,and the relative standard deviations(RSD) of the method for precision and reproducibility were less than 3.0%.The eight components were found to be stable for 24 h in room temperature,and there existed good linear relationships for the analytes in corresponding mass concentration ranges with their correlation coefficients(r) not less than 0.999 5.The limits of detection and the limits of quantitation were in the ranges of 0.40-10.58 μg·mL-1 and 1.31-34.61 μg·mL-1,respectively.Average recoveries for the 8 components were between 97.3%and 108%with RSD(n=6) of 0.51%-3.2%.Therefore,this method could be applied in the quantitative analysis of the 8 components in Hedera helix L..
Key Words:Hedera helix L.  ultra high performance liquid chromatography(UHPLC)  ivy saponins  organic acids  flavonoids
引用本文:倪力军,文丹瑶,胡甜甜,高丽丽,胡江宁,金汉台,金志文,张立国.超高效液相色谱法同时测定洋常春藤中8种成分含量[J].分析测试学报,2019,38(11):1369-1373.
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