| Paternò-Büchi(PB)反应与串联质谱结合实现不饱和脂质精确结构解析 |
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| 基金项目:国家自然科学基金项目(21722506，21705091) |
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| 中文摘要:脂质在一系列关键生命过程中发挥着重要作用。结构决定功能，因此脂质结构解析与功能研究一直受到研究者的关注。尽管质谱已成为脂质分析的最常用技术之一，但脂质的精细结构解析仍面临诸多挑战。为解决上述问题，将Paternò-Büchi(PB)光化学反应与串联质谱相结合，实现了不饱和脂质中碳碳双键(C‖C)的定位以及不饱和脂质C‖C位置异构体的定量分析。此外，该技术可与鸟枪法、液相色谱-质谱和质谱成像等技术联用，用于复杂生物样品中脂质的分析。 |
| 中文关键词:脂质 串联质谱 光化学衍生 结构解析 |
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| Comprehensive Structural Characterization of Unsaturated Lipids via Paternò-Büchi(PB) Reactions and Tandem Mass Spectrometry |
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| Abstract:Lipids play important roles in a variety of critical biological processes.Therefore,significant research efforts have been devoted to the structural characterization and functional analysis of lipids.Although mass spectrometry(MS) has become one of the most common techniques for rapid and sensitive analysis of lipids,the structural characterization on lipids remains to be an analytical challenge.Aiming to solve this problem,an effective and widely adopted method was developed for localizing C‖C(s) in unsaturated lipids and quantifying lipid C‖C location isomers.Besides,this method could be readily coupled with shotgun lipidomics,liquid chromatography-mass spectrometry(LC-MS) and MS imaging,to achieve detailed lipid structural analysis on various types of biological samples. |
| Key Words:lipids tandem mass spectrometry photochemical derivatization structural elucidation |
| 引用本文：马潇潇，胡清源，瑕瑜.Paternò-Büchi(PB)反应与串联质谱结合实现不饱和脂质精确结构解析[J].分析测试学报,2020,39(1):19-27. |
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