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黄芩-栀子配伍治疗糖尿病肾病大鼠的尿液代谢组学研究
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作者单位
程晓旭,门丽慧,皮子凤,宋凤瑞,刘志强 1.中国科学院长春应用化学研究所长春质谱中心吉林省中药化学与质谱重点实验室2.中国科学技术大学应用化学与工程学院3.吉林大学基础医学院 
基金项目:国家自然科学基金项目(81473537,81373952)
中文摘要:采用基于超高效液相色谱与串联四极杆飞行时间质谱仪(Ultra high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry,UHPLC-Q-TOF MS)联用的代谢组学方法,通过分析大鼠尿液内源性代谢物的变化,研究黄芩-栀子药对治疗糖尿病肾病的作用机制。利用高脂高糖饲料喂养并腹腔注射链脲佐菌素(STZ)建立糖尿病大鼠模型。给药12周后,采用UHPLC-Q-TOF MS对正常对照组、模型组、黄芩-栀子药对治疗组的尿液样品进行代谢轮廓分析。利用正交偏最小二乘判别分析(Orthogonal partial least squares discriminant analysis,OPLS-DA)对实验数据进行分析,挑选出对各组分分离贡献较大(VIP>1,p<0.05)的化合物在Human Metabolome Database(HMDB)等数据库进行质谱信息匹配,共鉴定出糖尿病肾病潜在生物标记物31个,给药后回调生物标记物16个。研究结果表明,黄芩-栀子药对通过影响胆汁酸的合成与代谢、尿毒素代谢、能量代谢、色氨酸代谢、苯丙氨酸代谢、酪氨酸代谢等通路对糖尿病肾病起到治疗作用,其中胆汁酸的合成与代谢、尿毒素代谢和能量代谢可能是黄芩-栀子药对发挥治疗作用的主要途径。与单味药相比,配伍后的药对具有了新的药理作用。
中文关键词:黄芩-栀子药对  黄芩  栀子  糖尿病肾病  尿液
 
Urinary Metabolomics Study of Rat Suffering Diabetic Nephropathy Treated with Radix Scutellariae-Gardenia jasminoides Couples
Abstract:A urinary metabonomics method based on ultra high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry(UHPLC-Q-TOF MS) was established for the investigation on mechanism of Radix Scutellariae-Gardenia jasminoides couples treating diabetic nephropathy.A diabetic model was established by streptozotocin intraperitoneal injection and feeding with high fat and high sucrose chow.After intragastric administration for 12 weeks,UHPLC-Q-TOF MS was used to analyze the metabolic profiles of the urinary samples from the control group,the model group and the Radix Scutellariae-Gardenia jasminoides Couples treatment group,which were then compared with the results from the Radix Scutellariae treatment group and the Gardenia jasminoides treatment group.The data were analyzed by Orthogonal partial least-squares discriminant analysis(OPLS-DA).According to the results of OPLS-DA,the compounds providing greater contribution to separation of each group were searched from the HMDB databases.Finally,31 potential biomarkers were identified,and 16 of them were related to the RadixScutellariae-Gardenia jasminoides Couples.Results revealed that Radix Scutellariae-Gardenia jasminoides couples played a role in diabetic nephropathy treatment through mainly affecting the pathways of tryptophan metabolism,phenylalanine metabolism,tyrosine metabolism,energy metabolism and biosynthesis of bile acids,in which biosynthesis of bile acids,urotoxin metabolism and energy metabolism are the important pathways to treat diabetic nephropathy.Compared with the single drug of Radix Scutellariae or Gardenia jasminoides,Radix Scutellariae-Gardenia jasminoides couples had the special treatment effect.
Key Words:Radix Scutellariae-Gardenia jasminoides couples  Radix Scutellariae  Gardenia jasminoides  diabetic nephropathy  urine
引用本文:程晓旭,门丽慧,皮子凤,宋凤瑞,刘志强.黄芩-栀子配伍治疗糖尿病肾病大鼠的尿液代谢组学研究[J].分析测试学报,2020,39(1):128-135.
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